Abstract
The photosynthetic membrane of chloroplasts is differentiated into appressed and non-appressed (stroma exposed) areas. This structural differentiation is accompanied by a functional heterogeneity in the lateral plane of the membrane1. The photosystem 1 complex and the ATP synthase are mainly excluded from the appressed membrane regions and localized in the non-appressed regions. Most of the photosystem 2 complexes and its light-harvesting chlorophyll a/b complex (LHC-II) is located in the appressed regions. This lateral segregation of the thylakoid protein complexes has been postulated to involve repulsive electrostatic and attractive van der Waals forces at closely appressed membrane surfaces2. Under low salt conditions the thylakoid membranes destack and the membrane components are randomized along the membrane3. Readdition of cations reverses this process and restacking accompanied by lateral segregation of the complexes occurs.
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References
J.M. Anderson and B. Andersson, The architecture of photosynthetic membranes: lateral and transverse organization, Trends Biochem. Sci. 7: 288 (1982)
J. Barber, Influence of surface charges on thylakoid structure and function, Annu. Rev. Plant Physiol. 33: 261 (1982)
L.A. Staehelin, Reversible particle movements associated with un-stacking and restacking of chloroplast membranes in vitro, J. Cell. Biol. 71: 136 (1976)
L.A. Staehelin and C.J. Arntzen, Regulation of chloroplast membrane function: protein phosphorylation changes the spatial organization of membrane components, J. Cell Biol. 97: 1327 (1983).
P.V. Sane, D.J. Goodchild and R.B. Park, Characterization of chloroplast photosystem 1 and 2 separated by a non-detergent method, Biochim. Biophys. Acta 216: 162 (1970).
B. Andersson, C. Sundby, H.-E. Akerlund and P.-A. Albertsson, Inside-out thylakoid vesicles: important tools for the characterization of the photosynthetic membrane, Physiol. Plant. in press (1985).
B. Andersson, C. Sundby and P.-A. Albertsson, A mechanism for the formation of inside-out membrane vesicles, Biochim. Biophys. Acta 599: 391 (1980).
B. Andersson and H.-E. Akerlund, Inside-out membrane vesicles isolated from spinach thylakoids, Biochim. Biophys. Acta 503: 462 (1978).
D.J. Arnon, Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris, Plant Physiol. 24: 1 (1949).
J.M. Anderson, J.C. Waldron and S.W. Thorne, Chlorophyll-protein complexes of spinach and barley thylakoids, FEBS Lett. 92: 227 (1978).
J.E. Mullet and G.J. Arntzen, Simulation of grana stacking in a model membrane system, Biochim. Biophys. Acta 589: 100 (1980).
I.J. Ryrie, Freeze-fracture analysis of membrane appression and protein segregation in model membranes containing the chlorophyll-protein complexes from chloroplasts, Eur. J. Biochem. 137: 203 (1983).
J.M. Briantais, C. Vernotte, J. Olive and F.-A. Wollman, Kinetics of cation-induced changes of photosystem II fluorescence and of lateral distribution of the two photosytems in the thylakoid membranes of pea chloroplasts, Biochim. Biophys. Acta 766: 1 (1984).
D.J. Kyle, L.A. Staehelin and C.J. Arntzen, Lateral mobility of the light-harvesting complex in chloroplast membranes controls excitation energy distribution in higher plants, Arch. Biochem. Biophys. 222: 527 (1983).
L.A. Staehelin and M. DeWit, Correlation of structure and function of chloroplast membranes at the supramolecular level, J. Cell Biochem. 24: 261 (1984).
P. Rand, Interacting phospholipid bilayers: Measured forces and induced structural changes, Annu. Rev. Biophys. Bioeng. 10: 277 (1981).
J.T. Duniec, J.N. Israelachvilli, B.W. Ninham, R.M. Pashley and S.W. Thorne, An ion-exchange model for thylakoid stacking in chloroplasts, FEBS Lett. 129: 193 (1981).
H.-E. Akerlund, B. Andersson, A. Persson and P.-A. Albertsson, Isoelectric points of spinach thylakoid membrane surfaces as determined by cross partition, Biochim. Biophys. Acta 552: 238 (1979).
P.D. Gerola, R.C. Jennings, G. Forti and F.M. Garlaschi, Influence of protons on thylakoid membrane stacking, Plant Sci. Lett. 16: 249 (1979).
J. Barber, An explanation for the relationship between salt-induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from photosystem 1 to photosystem 11, FEBS Lett. 118: 1 (1980).
J. Bennet, Phosphorylation of chloroplast membrane polypeptides, Nature 269: 344 (1977).
U.K. Larsson, B. Jergil and B. Andersson, Changes in the lateral distribution of the light-harvesting chlorophyll-a/b-protein complex induced by its phosphorylation, Eur. J. Biochem. 136: 25 (1983).
D.J. Kyle, T.-Y. Kuang, J.L. Watson and C.J. Arntzen, Movement of a sub-population of the light-harvesting complex (LHC-II) from grana to stroma lamellae as a consequence of its phosphorylation, Biochim. Biophys. Acta 765: 89 (1984).
A. Telfer, M. Hodges, P.A. Millner and J. Barber, The cation-dependence of the degree of protein phosphorylation-induced unstacking of pea thylakoids, Biochim. Biophsy. Acta 766: 554 (1984).
F.-A. Wollman and B.A. Diner, Cation-control of fluorescence emission, light scatter and membrane stacking in pigment mutants of Chlamydomonas reinhardi, Arch. Biochem. Biophys. 201: 646 (1980).
J.H. Argyroudi-Akoyunoglou, Effect of cations on the reconstitution of heavy subchloroplast fractions (grana) in disorganized low-salt agranal chloroplasts, Arch. Biochem. Biophys. 176: 267 (1976).
W.S. Chow and J. Barber, Further studies of the relationship between cation-induced chlorophyll fluorescence and thylakoid membrane stacking changes, Biochim. Biophys. Acta 593: 149 (1980).
H.-E. Akerlund, B. Andersson and P.-A. Albertsson, Isolation of photosystem II enriched membrane vesicles from spinach chloroplasts by phase partition, Biochim. Biophys. Acta 449: 525 (1976).
C. Tanford, “The hydrophobic effect”, Wiley-Interscience, New York (1973).
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© 1986 Plenum Press, New York
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Sundby, C., Larsson, U.K., Andersson, B. (1986). Ionic Effects on the Lateral Segregation of Chlorophyll-Proteins during Restacking of Thylakoid Membranes. In: Papageorgiou, G.C., Barber, J., Papa, S. (eds) Ion Interactions in Energy Transfer Biomembranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8410-6_25
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DOI: https://doi.org/10.1007/978-1-4684-8410-6_25
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