Abstract
The membrane-bound ATPase in chloroplasts can catalyze ATP synthesis/hydrolysis coupled with a transmembrane proton transport. The detailed mechanism by which proton transport gives rise to the formation of a phosphate-anhydrid bond is still not known. We have investigated the kinetics of ATP synthesis with chloroplasts and with CFo F1 reconstituted into liposomes. From these results we want to draw conclusions about the mechanism of the reaction. The rate of ATP synthesis was measured with a rapid double-mixing system using artificially impressed transmembrane pH difference, ∆ pH, electric potential difference, ∆Ψ and phosphate potential, ∆Gp (1).
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© 1986 Plenum Press, New York
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Gräber, P., Junesch, U., Schmidt, G., Fromme, P. (1986). Proton Transport-Coupled ATP Synthesis Catalyzed by the Chloroplast ATPase. In: Papageorgiou, G.C., Barber, J., Papa, S. (eds) Ion Interactions in Energy Transfer Biomembranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8410-6_15
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DOI: https://doi.org/10.1007/978-1-4684-8410-6_15
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