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Biochemical Characterization of Natural Killer Cytotoxic Factors

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Mechanisms of Cell-Mediated Cytotoxicity II

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 184))

Abstract

Earlier studies of the NK lytic mechanism produced evidence for the stimulus secretion model and led to the postulation that lytic mediators are transferred from the effector to the target cell (1). Subsequent work in our laboratory demonstrated that soluble natural killer cytotoxic factors (NKCF) can be detected in the supernatants of effector cells stimulated with NK targets or with lectin (2,3). Further experiments were performed to analyze the functional characteristics of these factors. It was found that NKCF are released from human PBL (4) or murine spleen cells (2) that bear the phenotypic characteristics of NK cells. Release of NKCF is inhibited by some of the same protease inhibitors (5) shown previously to inhibit NK CMC (6). Pretreatment of effector cells with interferon enhances the release of NKCF (7) whereas interferon pretreatment of YAC-1 cells impairs their ability to stimulate release of the factors (8), thus accounting for the dual effects of interferon on NK CMC (9). NKCF lyse only NK sensitive targets (2,4) and YAC-1 variants selected for resistance to NKCF are also resistant to NK CMC (10).

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA, USA

    Susan C. Wright, Stanley M. Wilbur & Benjamin Bonavida

  2. Department of Microbiology, University of Southern California, Los Angeles, CA, USA

    Stanley M. Wilbur

Authors
  1. Susan C. Wright
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  2. Stanley M. Wilbur
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  3. Benjamin Bonavida
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Editors and Affiliations

  1. National Institutes of Health, Bethesda, Maryland, USA

    Pierre Henkart

  2. University of Massachusetts, Amherst, Massachusetts, USA

    Eric Martz

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© 1985 Plenum Press, New York

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Wright, S.C., Wilbur, S.M., Bonavida, B. (1985). Biochemical Characterization of Natural Killer Cytotoxic Factors. In: Henkart, P., Martz, E. (eds) Mechanisms of Cell-Mediated Cytotoxicity II. Advances in Experimental Medicine and Biology, vol 184. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8326-0_12

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  • DOI: https://doi.org/10.1007/978-1-4684-8326-0_12

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