An Experimental Set-Up for the Blood Perfused Working Isolated Rat Heart
The isolated working heart preparation, first described by Neely et al. (1967), has now been used for over two decades to assess myocardial performance and left ventricular function in response to various disease states, drug treatments etc. However, usually a well oxygenated electrolyte solution is used as perfusion medium, which makes the oxygenation of these hearts very unphysiological. The present study reports on an experimental set-up for the isolated working heart model that can be used to study myocardial oxygenation. The perfusion system is a modification of that described by Duvelleroy et al. (1976) for perfusion of the heart with an erythrocyte suspension in buffer. A microcomputer data acquisition and analysis system was developed to avoid time consuming data evaluation by hand. With this set-up, important hemodynamic variables like myocardial oxygen consumption, cardiac work and maximal positive and negative first derivative of the left ventricular pressure can be calculated on line. Our aim is to use this set-up for the evaluation of the mathematical modeling work of our group on tissue oxygenation (e.g. Turek et al., in press; Hoofd et al, this volume) by varying the various input parameters concerning the oxygen supply of the hearts.
KeywordsCoronary Flow Aortic Pressure Left Ventricular Pressure Myocardial Oxygen Consumption Retrograde Perfusion
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