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A Method to Measure the Diffusion Coefficient of Myoglobin in Intact Skeletal Muscle Cells

  • Klaus D. Jürgens
  • Thomas Peters
  • Gerolf Gros
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 277)

Abstract

A new method was developed to measure the diffusion coefficient of myoglobin in intact muscle cells, a quantity that allows one to estimate the contribution of myoglobin to facilitated oxygen transport. So far, the diffusion coefficient of myoglobin in mammalian muscle cells has been estimated from results obtained from muscle homogenates (Moll, 1968) or from measurements performed on frog muscle cells, into which myoglobin was injected (Baylor and Pape, 1988). With these data no reliable estimate can be made of the extent to which myoglobin diffusion facilitates oxygen transport within mammalian muscle cells.

Keywords

Absorbance Spectrum Absorbance Change Soret Band Diaphragm Muscle Muscle Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baylor, S.M. and Pape, P.C., 1988, Measurement of myoglobin diffusivity in the myoplasm of frog skeletal muscle fibers, J. Physiol., 406:247.PubMedGoogle Scholar
  2. Crank, J., 1956, “The Mathematics of diffusion”, Oxford University Press, London.Google Scholar
  3. Demma, L.S., Salhany, J.M., 1977, Direct generation of superoxide anions by flash photolysis of human oxyhemoglobin, J. Biol. Chem., 252:1226.PubMedGoogle Scholar
  4. Moll, W., 1968, The diffusion coefficient of myoglobin in muscle homogenate, Pflüg. Arch., 299:247.CrossRefGoogle Scholar
  5. Riveros-Moreno, V. and Wittenberg, J.B., 1972, The self-diffusion coefficients of myoglobin and hemoglobin in concentrated solutions, J. Biol. Chem., 247:895.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Klaus D. Jürgens
    • 1
  • Thomas Peters
    • 1
  • Gerolf Gros
    • 1
  1. 1.Zentrum PhysiologieMedizinische HochschuleHannoverGermany

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