Abstract
Precise knowledge of the oxygen diffusion coefficient (D) in tissue is essential for the analysis of oxygen transport. The PO2 profile in a tissue can be measured with a deeply recessed (L/d > 5), Polarographic microelectrode (Linsenmeier, 1986; Haugh et al., 1990). By contrast, one with a shallow recess (L/d < 1) is affected by D in the tissue. It is possible to utilize this feature to determine the tissue D from the polarization transient when the electrode is turned on (i.e., the imposed voltage is changed). The faster the approach to steady state, the larger the value of D must be. Previous attempts to utilize a microelectrode turn-on transient to give local values of D in tissue (Erdmann and Krell, 1976; Buerk, 1980; Buerk and Goldstick, 1990) were plagued by the problem that initially the extremely large current saturated the amplifier. In the present study, the amplifier saturation was eliminated by open-circuiting the amplifier for a few ms initially. All of the data recorded after the initial delay, including those at very early times, were used for the calculation of D by a nonlinear regression analysis. Apparently there have been no reliable previous measurements of D in the mammalian retina or cornea in situ. The usual previous approach has been to make the measurement on a slice of excised tissue in vitro. The measurements in the present study are therefore novel because they are local and made in situ in the intact tissue of a living animal.
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References
Briggs, D. and Rodenhaeuser, J.-H., 1973, Distribution and consumption of oxygen in the vitreous body of cats, in: “Oxygen Supply: Theoretical and Practical Aspects of Oxygen Supply and Microcirculation of Tissue”, M. Kessler, D.F. Bruley, L. C. Clark, Jr., D.W. Luebbers, I. A. Silver, J. Strauss, eds., Urban and Schwarzenberg, Munich, pp. 265–269.
Buerk, D.G., 1980, “Hypoxia in the Walls of Large Blood Vessels” (PhD thesis), Northwestern University, Evanston, IL, USA.
Buerk, D.G. and Goldstick, T.K., 1990, Oxygen diffusion coefficient determined from Polarographic oxygen electrode turn-on transients: Spatial variation of diffusivity in the aortic wall, In preparation.
Erdmann, W. and Krell, W., 1976, Measurements of diffusion with noble metal electrodes, Adv. Exp. Med. Biol. 75: 225–228.
Freeman, R.D. and Fatt, I., 1972, Oxygen permeability of the limiting layers of the cornea, Biophys. J., 12: 237–247.
Goldstick, T.K. and Fatt, I., 1970, Diffusion of oxygen in solutions of blood proteins, Chem. Eng. Prog. Symp. Ser. No. 99, 66: 101–113.
Grote, J. and Zander, R., 1976, Corneal oxygen supply conditions, Adv. Exp. Med. Biol, 75: 449–455.
Haugh, L.M., Linsenmeier, R.A., and Goldstick, T.K., 1990, Mathematical models of the spatial distribution of retinal oxygen tension and consumption, including changes upon illumination, Annals Biomed. Eng., 18: 19–36.
Heald, K. and Langham, M.E., 1956, Permeability of the cornea and blood-aqueous barrier to oxygen, Brit. J. Ophthal, 40: 705–720.
Kakihana, M., Ikeuchi, H., Sato, G.P., and Tokuda, K., 1981, Diffusion current at microdisk electrodes — Application to accurate measurement of diffusion coefficients, J. Electroanal Chem., 117: 201–211.
Laitinen, H.A. and Kolthoff, I.M., 1939, A study of diffusion processes by electrolysis with microelectrodes, J. Am. Chem. Soc., 61: 3344–3349.
Linsenmeier, R.A., 1986, Effects of light and darkness on oxygen distribution and consumption in the cat retina, J. Gen. Physiol., 88: 521–542.
Linsenmeier, R.A., Goldstick, T.K., Rlum, R.S., and Enroth-Cugell, C., 1981, Estimation of retinal oxygen transients from measurements made in the vitreous humor, Exp. Eye Res., 32: 369–379.
Linsenmeier, R.A., Goldstick, T.K., and Zhang, S.-L., 1989, Chinese herbal medicine increases tissue oxygen tension, Adv. Exp. Med. Biol., 248: 795–801.
Linsenmeier, R.A. and Yancey, C.M., 1987, Improved fabrication of double-barreled recessed cathode O2 microelectrodes, J. Appl. Physiol., 63: 2554–2557.
Maurice, D.M., 1984, The cornea and sclera, in: “The Eye”, H. Davson, ed., 3rd ed., vol 1b, Academic Press, New York, p. 12.
Parker, K.H. and Winlove, C.P., 1983, The measurement of oxygen diffusivity and solubility in tissue using a transient Polarographic technique, J. Physiol (London), 341: 45P-46P.
Roh, H.-D., 1989, “Local Oxygen Diffusion Coefficients in the Cat Retina and Cornea” (PhD thesis), Northwestern University, Evanston, IL, USA.
Soo, Z.G. and Lingane, J., 1964, Derivation of the chronoamperometric constant for unshielded, circular, planar electrodes, J. Phys. Chem., 68: 3821–3828.
Takahashi, G.H. and Fatt, I., 1965, The diffusion of oxygen in the cornea, Exp Eye Res., 4: 4–12.
Takahashi, G.H., Fatt, I., and Goldstick, T.K., 1966, Oxygen consumption rate of tissue measured by a micropolarographic method, J. Gen. Physiol., 50: 317–335.
Vaupel, P., 1976, Effect of percentual water content in tissue and liquids on the diffusion coefficient of O2, CO2, and H2, Pfluegers Arch., 361:201–204.
Winlove, C.P. and Parker, K.H., 1984, The measurement of oxygen diffusivity and concentration by chronoamperometry using microelectrodes, J. Electroanal. Chem., 170: 293–304.
Yancey, C.M. and Linsenmeier, R.A., 1989, Oxygen distribution and consumption in the cat retina at increased intraocular pressure, Invest Ophthalmol Visual. Sci., 48: 600–611.
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© 1990 Plenum Press, New York
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Roh, HD., Goldstick, T.K., Linsenmeier, R.A. (1990). Spatial Variation of the Local Tissue Oxygen Diffusion Coefficient Measured in situ in the Cat Retina and Cornea. In: Piiper, J., Goldstick, T.K., Meyer, M. (eds) Oxygen Transport to Tissue XII. Advances in Experimental Medicine and Biology, vol 277. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8181-5_17
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DOI: https://doi.org/10.1007/978-1-4684-8181-5_17
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