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Neutrophil Migration and Orientation Under Agarose: Findings in Patients with the Immotile Cilia Syndrome and Effects of Cytochalasin B and Vinblastine

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Biochemistry and Function of Phagocytes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 141))

Abstract

The technique measuring the migration of PMN neutrophil granulocytes under agarose is simple and inexpensive. Usually results are expressed as the distance migrated by the leading front cellsl, but it is also possible to quantitate number of migrating cells2,3 and observe their morphology4. Finally, modifications have been presented for discriminating chemokinesis (enhanced random migration) from chemotaxis (directed migration) by a checker-board analysis2, or by incorporation of migration stimulating agents, cytotaxins, into the agarose5.

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References

  1. R.D. Nelson, P.D. Quie and R.L. Simmons, Chemotaxis under agarose: a new and simple method, J. Immunol. 115: 1650 (975).

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Authors and Affiliations

  1. Depts. of Medicine IV & Clin. Bact., the Karolinska Institute at Södersjukhuset, S-10064, Stockholm, Sweden

    Jan Palmblad, Ann-Mari Udén, Nikos Venizelos & Björn Afzelius

  2. Wennergren Institute, Univ. of Stockholm, Sweden

    Jan Palmblad, Ann-Mari Udén, Nikos Venizelos & Björn Afzelius

Authors
  1. Jan Palmblad
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  2. Ann-Mari Udén
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  3. Nikos Venizelos
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  4. Björn Afzelius
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  5. H. U. Keller
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Editor information

Editors and Affiliations

  1. Instituto di Patologia Generale dell’ Università di Padova, Verona, Italy

    Filippo Rossi

  2. Instituto di Patologia Generale dell’ Università degli Studi di Trieste, Trieste, Italy

    Pierluigi Patriarca

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© 1982 Plenum Press, New York

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Palmblad, J., Udén, AM., Venizelos, N., Afzelius, B., Keller, H.U. (1982). Neutrophil Migration and Orientation Under Agarose: Findings in Patients with the Immotile Cilia Syndrome and Effects of Cytochalasin B and Vinblastine. In: Rossi, F., Patriarca, P. (eds) Biochemistry and Function of Phagocytes. Advances in Experimental Medicine and Biology, vol 141. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8088-7_6

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  • DOI: https://doi.org/10.1007/978-1-4684-8088-7_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8090-0

  • Online ISBN: 978-1-4684-8088-7

  • eBook Packages: Springer Book Archive

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