Abstract
Bovine neutrophils are a convenient material for biochemical studies since they can be obtained easily in large quantities. We report here some of the biochemical and functional properties of these cells. In contrast to the neutrophils thus far described, bovine neutrophils contain three distinct types of cytoplasmic storage granules and exhibit powerful oxygen-independent antibacterial properties.
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References
Carlson, G.P. and J.J.Kaneko. 1973. Isolation of leukocytes from bovine peripheral blood. Proc. Soc. Exp. Biol. Med. 142: 853.
Gennaro, R., C.Schneider, G.de Nicola, F.Cian, and D.Romeo. 1978. Biochemical properties of bovine granulocytes, Proc. Soc. Exp. Biol. Med. 157: 342.
Mottola, C., R.Gennaro, A.Marzullo and D.Romeo. 1980. Isolation and partial characterization of the plasma membrane of purified bovine neutrophils. Eur. J. Biochem. 111: 341.
Gennaro, R. and D.Romeo. 1979. The release of superoxide anion from granulocytes: Effect of inhibitors of anion permeability. Biochem. Biophys. Res. Commun. 88: 1979.
Bainton, D.F., J.L.Ullyot, and M.G.Farquhar. 1971. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. Origin and content of azurophil and specific granules. J. Exp. Med. 134: 907.
Baggiolini, M., J.G.Hirsch and C.de Duve. 1969. Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation. J. Cell Biol. 40: 529.
Bretz, U. and M.Baggiolini. 1974. Biochemical and morphological characterization of azurophil and specific granules of human neu-trophilic polymorphonuclear leukocytes. J. Cell Biol. 63: 251.
Baggiolini, M. 1972. The enzyme of the granules of polymorphonuclear leukocytes and their functions. Enzyme 13: 132.
Odeberg, H. and I.Olsson. 1975. Antibacterial activity of cationic proteins from human granulocytes. J. Clin. Invest. 56: 1118.
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© 1982 Plenum Press, New York
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Gennaro, R., Romeo, D., Dewald, B., Baggiolini, M., Clark, R.A. (1982). The Bovine Neutrophil: Separation and Partial Characterization of Plasma Membrane and Cytoplasmic Granules. In: Rossi, F., Patriarca, P. (eds) Biochemistry and Function of Phagocytes. Advances in Experimental Medicine and Biology, vol 141. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8088-7_29
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DOI: https://doi.org/10.1007/978-1-4684-8088-7_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8090-0
Online ISBN: 978-1-4684-8088-7
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