Summary
High performance liquid chromatography (HPLC) is the most widely used method for the separation of ecdysteroids. Many efficient methods are presently available, which are adapted to the various classes of compounds (high, medium or low polarity ecdysteroids), for either analytical or preparative purposes.
This review includes a survey of classical chromatographic systems, including some recent developments of normal-phase HPLC (concerning especially the use of polar and non-polar bonded silica columns), a few general comments on rather sophisticated detection systems (diode-array detectors, radioactivity monitors) and some comments regarding the problem of sample preparation prior to HPLC analysis.
Several possible future developments of HPLC, including coupling with mass spectrometry, are discussed briefly.
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Lafont, R., Wilson, I.D. (1990). Advances in Ecdysteroid High Performance Liquid Chromatography. In: McCaffery, A.R., Wilson, I.D. (eds) Chromatography and Isolation of Insect Hormones and Pheromones. Chromatographic Society Symposium Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8062-7_8
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DOI: https://doi.org/10.1007/978-1-4684-8062-7_8
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