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Isolation and Identification of a Sulfakinin-Like Peptide, with Sequence Homology to Vertebrate Gastrin and Cholecystokinin, from the Brain of Locusta Migratoria

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Chromatography and Isolation of Insect Hormones and Pheromones

Part of the book series: Chromatographic Society Symposium Series ((CSSS))

Summary

A neuropeptide (pGlu-Leu-Ala-Ser-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-NH2) with a blocked N-terminus and related to leucosulfakinins I and II from the Madeira roach has been isolated from an extract of 9000 brain complexes of Locusta migratoria. The peptide stimulates the motility of the cockroach hindgut. Biological activity was monitored during purification by high performance liquid chromatography by observing the myotropic effect of column fractions on the isolated hindgut of Leucophaea moderne.

The peptide, designated as locustasulfakinin or Lom-SK shares a common C-terminal decapeptide fragment with leucosulfakinin II and a common heptapeptide fragment with leucosulfakinin I, indicating the importance of the constituent amino acids for biological activity. Locust asulfakinin is the third invertebrate neuropeptide which exhibits sequence homologies with the hormonally active fraction of the vertebrate hormones cholecystokinin, human gastrin II and caerulin. Its intestinal myotropic activity is analogous to that of gastrin and cholecystokinin. The sequence homologies as well as the analogous myotropic activities suggest that gastrin/cholecystokinin-like peptides have a long evolutionary history, and probably play an important role in physiology.

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Author information

Authors and Affiliations

  1. Zoological Institute of the University, Naamsestraat 59, B-3000, Leuven, Belgium

    L. Schoofs

  2. Veterinary Toxicology and Entomology Research Laboratory, Agricultural Research Service, US Department of Agriculture, College Station, TX, 77841, USA

    M. Holman & A. De Loof

  3. Laboratories for Invertebrate Neuroendocrine Research, Department of Entomology, Texas A & M University, College Station, TX, 77843, USA

    T. Hayes

Authors
  1. L. Schoofs
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  2. M. Holman
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  3. T. Hayes
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  4. A. De Loof
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Editor information

Editors and Affiliations

  1. University of Reading, Reading, UK

    A. R. McCaffery

  2. ICI Pharmaceuticals, Macclesfield, Cheshire, UK

    I. D. Wilson

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© 1990 Plenum Press, New York

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Schoofs, L., Holman, M., Hayes, T., De Loof, A. (1990). Isolation and Identification of a Sulfakinin-Like Peptide, with Sequence Homology to Vertebrate Gastrin and Cholecystokinin, from the Brain of Locusta Migratoria. In: McCaffery, A.R., Wilson, I.D. (eds) Chromatography and Isolation of Insect Hormones and Pheromones. Chromatographic Society Symposium Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8062-7_22

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  • DOI: https://doi.org/10.1007/978-1-4684-8062-7_22

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-8064-1

  • Online ISBN: 978-1-4684-8062-7

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