Skip to main content
SpringerLink
Log in
Book cover

New Trends in Pharmacokinetics pp 125–147Cite as

In Vitro Imaging

  • Chapter

  • 289 Accesses

Part of the book series: NATO ASI Series ((NSSA,volume 221))

Abstract

In vitro imaging is a broad topic, with books written on each subtopic discussed in summary form in this chapter. Thus, the presentation cannot be comprehensive, but presents principles and examples in areas of current interest in pharmacokinetics in which we have had some experience. The discussion starts with front end devices for data acquisition, and then considers the different elements in the imaging chain. Autoradiography (ARG) systems are described in detail to indicate some of the techniques common to film and digital image analysis systems, as well as to illustrate selected biological applications. The discussion concludes with selected examples from oncology and cardiology research.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aas, M., Hnatowich, D.J., Mardirossian, G., Griffin T., Salimi A., Atcher R., Hines J., Brill A.B., 1990. Long-time Distribution of 114m-In Labeled Monoclonal Antibody (B72.3) in Mice Bearing Human Tumor Xenografts. Antibody, Immunoconj. and Radiopharm. 3: 57.

    Google Scholar 

  • Angelini F., Bellazzini R., Brez A., Massai M.M., 1988. Digital Imaging with Advanced Gaseous Detectors Developed at Pisa University. In: “Nuclear Instruments and Methods in Physics Research” (K. Siegbahn editor), Volume A269, No.2, Proceedings of International Symposium on the Use of Wire Chambers in Medical Imaging, Belgium, June 28–30, 1987 ( F. Deconinck, M. Defrise, and S. Tavernier, editors), page 430. North Holland, Amsterdam.

    Google Scholar 

  • Bergman K., and Tjalve H., 1977. Three-step Autoradiography of Organic Solvents and Plastic Monomers to Register Total Radioactivity, Non Volatile Metabolites, and Non-extractable Metabolites. Acta Pharm et Toxic. 41: Supp. 1, page 22.

    Google Scholar 

  • Braylan R.C., and Benson, N.A., 1989. Flow Cytometric Analysis of Lymphomas. Arch Pathol Lab Med 113: 627.

    PubMed  CAS  Google Scholar 

  • Argy R., 1977. Tissue Pieces from Whole Body Sections Used for Chromatographic Separation of Metabolites. Acta Pharm et Toxic. 41: Supp. 1, page 16.

    Google Scholar 

  • Dilmanian F.A., Weber D.A., Coderre J.A., Joel D.D., Shi K.-C., Meinken G.E., Som P., Tang Y.-N., Volkow N.D., Yee C., Brill A.B., Watanabe M., Inuzuka E., Oba K., Gerson R., Iida H., Hiruma A., 1990. A High-Resolution SPECT System Based on a Microchannel Plate Imager. IEEE Trans. Nucl. Sci. 37: 687.

    Article  Google Scholar 

  • Duflow H., Maenhaut W., and DeReuck J., 1989. Regional Distribution of Potassium, Calcium, and Six Trace Elements in Normal Human Brain. Neurochemical Res. 14: 1099.

    Article  Google Scholar 

  • Fay F.S., Carrington W., and Fogarty K.E., 1989. Three-Dimensional Molecular Distribution in Single Cells Analyzed Using the Digital Imaging Microscope. J. Microscopy. 153: 133.

    Article  CAS  Google Scholar 

  • Fiori C.E. and Newbury D.E., 1978. Artifacts Observed in Energy Dispersive X-Ray Spectrometry in Scanning Electron Microscopy. In “Scanning Electron Microscopy”, Volume I, page 401. Scanning EM Inc., AMF O’Hare, IL.

    Google Scholar 

  • Flessner M.F., Fenstermacher J.D., Blasberg R.G., and Dedrick R.L., 1985. Peritoneal Absorption of Macromolecules Studied by Quantitative Autoradiography. Am. J. Physiol 248: H26 - H32.

    PubMed  CAS  Google Scholar 

  • Garrecht B.M., and Chapman J.D., 1983. The labelling of EMT-6 Tumours in BALB/C Mice with C-14 Misonidazole. Brit. J. Radiol 56: 745.

    Article  PubMed  CAS  Google Scholar 

  • Geisler F.H., Jones K.W., Fowler J.S., Ktaner H.W., Wolf A.P., Kronkite E.P., Slatkin D.N., 1974. Deuterium Micromapping of Biological Samples Using the D(T,n)He-4 Reaction and Plastic Track Detectors. Science 186: 361.

    Article  PubMed  CAS  Google Scholar 

  • Green D., Howell G.R., Thorne M.C., and Watts R.H., 1978. Imaging of Tissue Sections on Lexan by Alpha-Particles and Thermal Neutrons: An aid in fissionable radionuclide distribution studies. Int. J. App. Rad. and Isotopes 29: 285.

    Article  CAS  Google Scholar 

  • Griffith M.H., Yorke E.D.,Wessels B.W., DeNardo G.L., Neacy W.P., 1988. Direct Dose Confirmation of Quantitative Autoradiography with Micro-TLD Measurements for Radioimmunotherapy. J. Nucl Med. 29: 1795.

    PubMed  CAS  Google Scholar 

  • Hainfeld J.F., 1987. A Small Gold-conjugated Antibody Label: Improved Resolution for Electron Microscopy. Science 236: 450–453.

    Article  PubMed  CAS  Google Scholar 

  • Harris L.D., Camp J.J., Ritman E.L. and Robb R.A., 1986. Three-dimensional Display analysis of Tomographic Volume Images Utilizing a Varifocal Mirror. IEEE Trans.Med.Imaging 5: 67.

    Article  PubMed  CAS  Google Scholar 

  • Hiraoka Y., Sedat J.W. and Agard D.A., 1987. The use of Charged Coupled Device for quantitative optical microscopy of biological structures. Science 238: 36.

    Article  PubMed  CAS  Google Scholar 

  • Ito T., and Brill A.B., 1990. Validity of Tissue Paste Standards for Quantitative Whole-Body Autoradiography Using Short-Lived Radionuclides. Appl. Radiat. Isot. 41: 661.

    Article  CAS  Google Scholar 

  • Ito T., Griffin T.W., Collins J.A., and Brill A.B., 1990. Immunotoxin Penetration into Tumor Foci Assessed by Quantitative Autoradiography. In: “Proceedings of Second International Symposium on Immunotoxins, June 14–16, 1990 Lake Buena Vista, FL.”

    Google Scholar 

  • Jones K.W., Gordon B.M., Hanson A.L., Kwiatek W.M., Pounds J.G., 1988. X-ray Fluorescence with Synchrotron Radiation. Ultramicroscopy 24: 313.

    Article  PubMed  CAS  Google Scholar 

  • Laskey, R.A., no date. Radioisotope Detection by Fluorography and Intensifying Screens. Rev. 23. Amersham Corp. Arlington Hts, IL.

    Google Scholar 

  • Lear J.L., Jones S.C., Greenberg J.H., Fedora T.J., Reivich M., 1981. Use of I123 and C-14 in a Double Radionucle Autoradiography Technique for Simultaneous Measurement of LCBF and LCMRgl. Stroke 12: 589.

    Article  PubMed  CAS  Google Scholar 

  • Lear J.L., Ackermann R., Kameyama M., Carson R., Phelps M., 1984. Multiple Radionuclide Autoradiography in Evaluation of Cerebral Function. J. Cer. Blood Flow and Metab. 4: 264.

    Article  CAS  Google Scholar 

  • Leichner P.K., Yang N.C., Frenkel T.L., Loudenslager D.M., Hawkins W.G., Klein J.L. and Order S.E., 1988. Dosimetry and Treatment Planning for Y-90 Labeled Antiferritin in Hepatoma. Int. J. Rad. Oncol, Biol, Physics. 14: 1033.

    Article  CAS  Google Scholar 

  • Loats H.L., Pittenger M., Unnerstall J.R., Loyd D.G. and Tucker R.W., 1988. Biomedical Image Analysis Applications. In: “Imaging Techniques in Biology and Medicine” (C. C Swenberg and J. J. Conklin, editors),.page 1. Academic Press, New York.

    Google Scholar 

  • Merkel D.E., Dressler L.G., McGuire W.L., 1987. Flow Cytometry, Cellular DNA Content and Prognosis in Human Malignancy. J. Clin. Oncol. 5: 1690.

    PubMed  CAS  Google Scholar 

  • Nadji M. and Morales A.R., 1984. Immunoperoxidase II: Practical Applications. Lab Medicine 15: 343.

    Google Scholar 

  • Ploem-Zaaijer J.J., Beyer-Boon M.E., Leyte-Veldstra L. and Ploem, J., 1979. Cytofluorometric and Cytophotometric DNA Measurements of Cervical Smears Stained using a new Bi-color Method. In: “The Automation of Cancer Cytology and Cell Image Analysis” ( N.J. Pressman and G.L. Wied, editors), page 225. Tutorials of Cytology, International Academy of Cytology, Chicago, IL.

    Google Scholar 

  • Pounds J.G., Long G.J., Kwiatek W.M., Jones K.W., Gordon B.M. and Hanson A.L., 1988. The Role of High Energy Synchrotron Radiation in Biomedical Trace Element Res. In: “X-Ray Microscopy” ( D. Sayre, M. Howells, J. Kirz, and H. Rarback, editors), Volume 2, page 425. Springer-Verlag, N.Y.

    Google Scholar 

  • Robb R.A. and Barillot C., 1989. Interactive Display and Analysis of 3-D. Images. IEEE Trans. Med. Imaging 8: 217.

    Article  CAS  Google Scholar 

  • Rogers A.W., 1979. “Techniques of Autoradiography”. Elsevier/North Holland Biomedical Press. Amsterdam.

    Google Scholar 

  • Seckinger D., Sugarbaker E., Frankfurt O., 1989. DNA Content in Human Cancer. Arch Pathol Lab Med 113: 619.

    PubMed  CAS  Google Scholar 

  • Shapiro H.M., 1989. Flow Cytometry of DNA Content and Other Indicators of Proliferative Activity. Arch Pathol Lab Med 113: 591.

    PubMed  CAS  Google Scholar 

  • Sonoda M., Takano M., Miyahara J. and Hisatoyo K., 1983. Computed Radiography utilizing Scanning Laser Stimulated Luminescence. Radiology 148: 833.

    PubMed  CAS  Google Scholar 

  • Stewart C.C., 1989. Flow Cytometric Analysis of Oncogene Expression in Human Neoplasia. Arch Pathol Lab Med 113: 634.

    PubMed  CAS  Google Scholar 

  • Taylor C.R., 1978. Immunoperoxidase Technique. Practical and Theoretical Aspects. Arch. Pathol. Lab. Med. 102: 113.

    PubMed  CAS  Google Scholar 

  • Taylor C.R., ed., 1986. “Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist”. Chapter 1, Principles of Immunomicroscopy. 1–22. W.B. Saunders Co., Philadelphia, PA.

    Google Scholar 

  • Ter-Pogosian M., Phelps M., Brownell G., Davis D. and Evens R., editors, 1977. “Reconstruction Tomography in Diagnostic Radiology and Nuclear Medicine”. University Park Press, Baltimore, MD

    Google Scholar 

  • Tubbs R.R. and Bauer T.W., 1989. Automation of Immunohistology. Arch Pathol Lab Med 113: 653.

    PubMed  CAS  Google Scholar 

  • Tuttle S.E., Jewell S.D., Mojzisik C.M., Hinkle G.H., Colcher D., Schlom J. and Martin E.W., 1988. Intraoperative Radioimmunolocalization of Colorectal Carcinoma with a Hand-held Gamma Probe and Mab B72.3: Comparison of In Vivo Gamma Probe Counts with In Vitro Mab Localization Int. J. Cancer 42: 352.

    Article  PubMed  CAS  Google Scholar 

  • Ullberg A., 1977. The Technique of Whole Body Autoradiography Cryosectioning of Large Specimens. Science Tools, LKB Inst. Journal, page 2.

    Google Scholar 

  • Unnerstall J.R., Kuhar M.J., Niehoff D.L. and Palacios J.M., 1981. Benzodiazepine Receptors are Coupled to a Subpopulation of Gamma-aminbu-tyric Acid (GABA) Receptors: Evidence from a Quantitative Autoradiographic Study. J. Pharmacol.Exp. Ther. 218: 797.

    PubMed  CAS  Google Scholar 

  • Venot A., Liehn J.-C., Lebruchec J.-F. and Roucayrol J.-C., 1986. Automated Comparison of Images. J. Nucl Med. 27: 1337.

    PubMed  CAS  Google Scholar 

  • Watson J.V., Sikora K. and Evan, G.I., 1985. A Simultaneous Flow Cytometric Assay for c-myc Oncoprotein and DNA in Nuclei from Paraffin-Embedded Material. J. Immunol. Methods. 83: 179.

    Article  PubMed  CAS  Google Scholar 

  • Wessels B.W., Yorke E.D. and Lambiotte, M., 1990. “Multicellular Level Dosi-metry and Low Dose Rate Effects. Frontiers in Nuclear Medicine. Dosimetry of Administered Radionuclides.” ACNP/SNM/DOE Publication, page 295.

    Google Scholar 

  • Yonekura Y., Brill A.B. Som P., Bennett G.W. and Fand I., 1983 a. Quantitative Autoradiography with Radiopharmaceutical. Part I. Digital Film Analysis System by Videodensitometry: Concise Communication. J. Nucl Med 24: 231.

    PubMed  CAS  Google Scholar 

  • Yonekura Y. and Brill A.B., 1983 b. Film Analysis Systems and Applications. In: “Digital Imaging in Medicine” (R. C. Reba, D. J. Goodenough, and H. F. Davidson,.editors), page 347. NATO ASI Series E. Applied Sciences No. 61. Martinus Nijhoff, The Hague.

    Google Scholar 

  • Yonekura Y., Brill A.B., Som P., Yamamoto K., Srivastava S. and Iwai J., 1985. Regional Myocardial Substrate Uptake in Hypertensive Rats: A Quantitative Autoradiographic Measurement. Science 227: 1494.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Nuclear Medicine and Radiology, University of Massachusetts Medical Center, Worcester, MA, 01655, USA

    A. Bertrand Brill & Andrew Karellas

Authors
  1. A. Bertrand Brill
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew Karellas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Parma, Parma, Italy

    Aldo Rescigno

  2. Hazleton Washington, Inc., Vienna, Virginia, USA

    Ajit K. Thakur

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Plenum Press, New York

About this chapter

Cite this chapter

Brill, A.B., Karellas, A. (1991). In Vitro Imaging. In: Rescigno, A., Thakur, A.K. (eds) New Trends in Pharmacokinetics. NATO ASI Series, vol 221. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8053-5_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-8053-5_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8055-9

  • Online ISBN: 978-1-4684-8053-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation