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Synthetic Hepatitis B Surface Antigen Peptide Vaccine

  • Gordon R. Dreesman
  • James T. Sparrow
  • Patrick J. Frenchick
  • Ronald C. Kennedy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 185)

Abstract

A synthetic hepatitis B surface antigen (HBsAg) peptide was prepared containing amino acid residues 122–137 of the major HBsAg polypeptide. This peptide was cyclized by the introduction of an intrachain disulfide bond between cysteine residues at positions 124 and 137 because previous studies had shown that intact disulfide bonds are critical for maintenance of HBsAg activity. An anti-HBs response was produced in mice by free peptide entrapped in liposomes. However, the immunogenicity was enhanced by aggregation into micelles, and by coupling to tetanus toxoid. Analysis of the peptide with a panel of monoclonal antibodies showed that peptide 122–137 contained a conformation (discontinuous) group a epitope and a sequential (continuous) subgroup y epitope. In addition, the cyclic peptide inhibited a human anti-HBs idiotype-antiidiotype reaction with specificity for group a determinant(s). The potential for synthetic peptides for hepatitis B virus vaccine development is discussed.

Keywords

Cyclic Peptide Tetanus Toxoid Aluminum Potassium Sulfate Intrachain Disulfide Bond Anhydrous Hydrogen Fluoride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HBsAg

hepatitis B surface antigen

HBcAg

hepatitis B core antigen

anti-HBs

antibodies to hepatitis B surface antigen

HBV

hepatitis B virus

SP1

synthetic hepatitis B surface antigen containing amino acid residues 122–137

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Gordon R. Dreesman
    • 1
  • James T. Sparrow
    • 2
  • Patrick J. Frenchick
    • 1
  • Ronald C. Kennedy
    • 1
  1. 1.Virology and Immunology DepartmentSouthwest Foundation for Biomedical ResearchSan AntonioUSA
  2. 2.Department of MedicineBaylor College of MedicineHoustonUSA

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