Immune Induction by a Protein Antigen and by a Peptide Segment of the Protein

  • Anne Marie Wan
  • Charles D. Estin
  • Beatrice C. Langton
  • Matthew L. Andria
  • Eli Benjamini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 185)


The immune induction by a protein (the tobacco mosaic virus protein — TMVP) was compared to the immune induction by the free, non-conjugated eicosa tryptic peptide fragment of the protein (tryptic peptide 8 representing residues 93–112 of the protein). The results demonstrated that like TMVP, peptide 8 was immunogenic in A/J mice. TMVP and peptide 8 do not cross react on the T cell level. However, immunization with TMVP or with peptide 8 induces antibodies which react with both TMVP and peptide 8. Characterization of the antibodies produced by both immunogens revealed that: 1) their isotope composition is similar with IgG1 and IgG2 being the predominant isotypes; this composition indicates that both immunogens are T cell dependent antigens, 2) the antibodies induced by TMVP and by peptide 8 are directed against the C-terminal decapeptide portion of peptide 8 (residues 103–112 of the protein), 3) the fine specificity of these antibodies is the same. These results, and results of adoptive transfer experiments, indicate that antigen specific T cells had no effect on the expression of the fine antibody specificity. The results demonstrate the feasibility of immunizing with a portion of a protein for the purpose of inducing antibodies with the same isotype composition and specificity towards a protein epitope as those induced by immunization with the whole protein.


Tobacco Mosaic Virus Helper Cell Tryptic Peptide Protein Antigen Fine Specificity 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Anne Marie Wan
    • 1
  • Charles D. Estin
    • 1
  • Beatrice C. Langton
    • 1
  • Matthew L. Andria
    • 1
  • Eli Benjamini
    • 1
  1. 1.Department of Medical Microbiology and Immunology School of MedicineUniversity of CaliforniaDavisUSA

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