Abstract
Identifying the factors responsible for the exquisite sensitivity of the brain to anoxia and ischemia has been very difficult with currently available preparations. During the past three years, our laboratory has utilized two fairly well characterized in vitro preparations to study the effects of anoxia on central mammalian neurons. The experiments described below, which employed cultures of dissociated hippocampal neurons and slices of rat hippocampus, implicate the synaptic release of glutamate (Glu) in the pathophysiology of anoxic neuronal death.
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References
Banker, G., and Cowan, W.M., 1977, Rat hippocampal neurons in dispersed cell culture, Brain Res. 126: 397.
Bartlett, W.P., and Banker, G.A., 1984, An electron microscopic study of the development of axons and dendrites by hippocampal neurons in culture. H. Synaptic relationships, J. Neurosci. 4:1954.
Bosley, T.M., Woodhams, P.L., Gordon, R.D., and Balázs, R., 1983, Effects of anoxia on the stimulated release of amino acid neurotransmitters in the cerebellum in vitro, J. Neurochem. 40:189.
Choi, D.W., 1985, Glutamate neurotoxicity in cortical cell culture is calcium-dependent, Neurosci. Lett. 58:293.
Clark, G., Samaie, M., and Rothman, S., 1985, Blockade of synaptic transmis- sion protects rat hippocampal slice from hypoxia, Ann. Neurol. 18:385.
Crunelli, V., Forda, S., and Kelly, J.S., 1983, Blockade of amino acid-induced depolarizations and inhibition of excitatory post-synaptic potentials in rat dentate gyrus, J. Physiol. 341:627.
Dingledine, R., 1983, N-methylaspartate activates voltage dependent calcium conductance in rat hippocampal pyramidal cells, J. Physiol. 343:385.
Jackson, M.D., Lecar, H., Brenneman, D.E., Fitzgerald, S., and Nelson, P.G., 1982, Electrical development in spinal cord cell culture, J. Neurosci. 2:1052.
Kass, I.S., and Lipton, P., 1982, Mechanisms involved in irreversible anoxic damage to the in vitro rat hippocampal slice, J. Physiol. 332:459.
Kuffler, S.W., Nicholls, J.G., and Martin, A.R., 1984, From Neuron to Brain. Sinauer, Sunderland, MA.
Lynch, G., and Baudry, M., 1984, The biochemistry of memory: a new and specific hypothesis, Science. 274: 1057.
Mayer, M.L., and Westbrook, G.L., 1984, Mixed-agonist action of excitatory amino acids on mouse spinal cord neurons under voltage clamp, J. Physiol. 354:29.
Olney, J.W., 1978, Neurotoxicity of excitatory amino acids, in: Kainic Acid as a Tool in Neurobiology. E.G. McGeer, J.W. Olney, and P.L. McGeer, eds., Raven, New York, p. 95.
Peacock, J.H., 1979, Electrophysiology of dissociated hippocampal cultures from fetal mice, Brain Res. 169: 247.
Peacock, J.H., Rush, D.F., and Mathers, L.H., 1979, Morphology of dissocia-ted hippocampal cultures from fetal mice, Brain Res. 169: 231.
Robinson, M.B., Anderson, K.D., Koerner, J.F., 1984, Kynurenic acid as an antagonist of hippocampal excitatory transmission, Brain Res. 309: 119.
Rosenberg, P.A., Schweitzer, J.S., and Dichter, M.A., 1984, Norepinephrine increases synaptic activity in rat cerebral cortex in dissociated cell culture, Soc. Neurosci. Abstr. 10:70.
Rothman, S.M., and Cowan, W.M., 1981, A scanning electron microscope study of the in vitro development of dissociated hippocampal cells, J. Comp. Neurol. 195:141.
Rothman, S.M., 1983, Synaptic activity mediates death of hypoxic neurons, Science. 270: 536.
Rothman, S., 1984, Synaptic release of excitatory amino acid neurotrans-mitter mediates anoxic neuronal death, J. Neurosci. 4:1884.
Rothman, S.M., 1985, The neurotoxicity of excitatory amino acids is produced by passive chloride influx, J. Neurosci. 5:1483.
Rothman, S.M., and Samaie, M., 1985, The physiology of excitatory synaptic transmission in cultures of dissociated rat hippocampus, J. Neurophvsiol. 54:701.
Rothman, S.M., and Olney, J.W., 1986, Glutamate and the pathophysiology of hypoxic/ischemic brain damage, Ann. Neurol. 19:105.
Segal, M., 1983, Rat hippocampal neurons in culture: responses to electrical and chemical stimuli, J. Neurophvsiol. 50:1249.
Silverstein, F.S., Buchanan, F., and Johnston, M.V., 1985, Hypoxia-ischemia gauses severe but reversible depression of striatal synaptosomal H-glutamate uptake, Ann. Neurol. 18:122.
Simon, R.P., Swan, J.H., Griffiths, T., and Melarum, B.S., 1984, Blockade of N-methyl-d-aspartate receptors may protect against ischemic damage in the brain, Science. 226: 850.
Thomson, A.M., West, D.C., and Lodge, D., 1985, An N-methylaspartate receptor-mediated synapse in rat cerebral cortex: a site of action of ketamine?, Nature. 313: 479.
Westbrook, G.C., and Mayer, M.L., 1984, Glutamate currents in mammalian spinal neurons: resolution of a paradox, Brain Res. 301: 375.
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© 1986 Plenum Press, New York
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Rothman, S.M. (1986). Glutamate and Anoxic Neuronal Death in Vitro . In: Schwarcz, R., Ben-Ari, Y. (eds) Excitatory Amino Acids and Epilepsy. Advances in Experimental Medicine and Biology, vol 203. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7971-3_52
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DOI: https://doi.org/10.1007/978-1-4684-7971-3_52
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