Abstract
Although the introduction of the patch-clamp techniques (Neher and Sakmann, 1976; Hamill et al., 1981) has been universally recognized as a major breakthrough in the biophysical study of ion channels, these techniques have not yet become a standard tool for pharmacological studies. We shall briefly describe below some of the results that we have obtained, using ‘outside-out’ patches of mouse central neurons (Nowak et al., 1984), in the characterization of excitatory amino acid (EAA) receptors and channels. In this description, we shall not develop the biophysical results (and in particular the description of kinetics of the channels) but shall concentrate on the results of direct pharmacological and physiological relevance.
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Ascher, P., Nowak, L., and Kehoe, J.S., 1986, Glutamate-activated channels in molluscan and vertebrate neurones, in: Ion Channels in Neural Membranes, J.M. Ritchie and R.D. Keynes, Alan R. Liss, in press.
Cull-Candy, S.G., and Ogden, D.C., 1985, Ion channels activated by L-glutamate and GABA in cultured cerebellar neurones of the rat, Proc. R, Soc. B., 224: 367.
Curtis, D.R., and Johnston, G.A.R., 1974, Amino acid transmitters in the mammalian central nervous system, Ergebn. Physiol., 69: 97.
Engberg, I., Flatman, J.A., and Lambert, J.D.C., 1979, The actions of excitatory amino acids on motoneurones in the feline spinal cord, J. Physiol., 288: 227.
Ferraro, T.N., and Hare, T.A., 1985, Free and conjugated amino acids in human CSF: influence of age and sex, Brain Res., 338: 53.
Foster, A.C., and Fagg, G.E., 1984, Acidic amino acid binding sites in mammalian neuronal membranes: their characteristics and relationship to synaptic receptors, Brain Res. Rev., 7: 101.
Foster, G.A., and Roberts, P.J., 1981, Kainic acid stimulation of cerebellar cyclic GMP levels: potentiation by glutamate and related amino acids, Neurosci. Lett., 23: 67.
Garthwaite, J., 1982, Excitatory amino acid receptors and guanosine 3’, 5’ cyclic monophosphate in incubated slices of immature and adult rat cerebellum, Neuroscience, 7: 2491.
Hamill, O.P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F., 1981, Improved patch clamp techniques for high resolution current recording from cells and cell-free membrane patches, Pflügers Arch., 391: 85.
Hamill, O.P., and Sakmann, B., 1981, Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells, Nature, 294: 462.
Hamill, O.P., Bormann, J., and Sakmann, B., 1983, Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA, Nature, 309: 160.
Mayer, M~, Westbrook, G., and Guthrie, P.B., 1984, Voltage dependent block by Mg of NMDA responses in spinal cord neurones, Nature, 309: 261.
McLennan, H., 1981, On the nature of receptors for various excitatory amino acids in the mammalian central nervous system, Adv. Biochem. Psychopharmacologv, 27: 253.
Neher, E., and Sakmann, B., 1976, Single channel currents recorded from membrane of denervated frog muscle fibers, Nature, 260: 799.
Nicoletti, F., Meek, J.L., Iadarola, M.J., Chuang, D.M., Roth, B.L., and Costa, E., 1986, Coupling of inositol phospholipid metabolism with excitatory amino acid recognition sites in rat hippocampus, J. Neurochem., 46: 40.
Nowak, L., Bregestovski, P., Ascher, P., Herbet, A., and Prochiantz, A., 1984, Magnesium gates glutamate-activated channels in mouse central neurones, Nature, 307: 462.
Sladeczek, F., Pin, J.P., Recasens, M., Bockaert, J., and Weiss, S., 1985, Glutamate stimulates inositol phosphate formation in striatal neurones, Nature, 317: 717.
Watkins, J.C., and Evans, R., 1981, Excitatory amino acid transmitters, Ann. Rev. Pharmacol. Toxicol., 21: 165.
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© 1986 Plenum Press, New York
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Ascher, P., Nowak, L. (1986). A Patch-Clamp Study of Excitatory Amino Acid Activated Channels. In: Schwarcz, R., Ben-Ari, Y. (eds) Excitatory Amino Acids and Epilepsy. Advances in Experimental Medicine and Biology, vol 203. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7971-3_39
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DOI: https://doi.org/10.1007/978-1-4684-7971-3_39
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