Abstract
We have been studying the genetic, molecular and developmental processes involved in the evagination of imaginal discs of Drosophila melanogaster. These studies have been facilitated, on the one hand, by the fact that evagination occurs in vitro in a variety of culture media in response to ecdysones (Mandaron, 1973; Chihara et al., 1972; Fristrom et al., 1973; Milner and Sang, 1974), and, on the other hand, by the availability of large numbers of imaginal discs (600,000–800,000 per day) isolated by a preparative procedure (Fristrom, 1972). Thus we have the advantage of both defined in vitro conditions and sufficient material for biochemical analysis. The morphology of an unevaginated and fully evaginated leg disc is compared in Fig. 1. Ecdysone-induced evagination is inhibited by all three of the known, naturally occurring juvenile hormones (JHs) and some, but not all, juvenile hormone analogs (Siegel and Fristrom, in press). In addition to inhibiting evagination, juvenile hormone also inhibits, under some conditions, DNA synthesis (Logan et al., 1975), RNA synthesis (Chihara and Fristrom, 1973) and protein synthesis, and affects some membrane properties (e.g., uridine transport, Chihara and Fristrom, 1973).
Previously unpublished work reported in this paper was supported in part by a grant from the National Institute of General Medical Sciences to J.W.F. (GM-19937).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ashburner, M., Chihara, C., Meltzer, P., and Richards, G., 1973, Cold Spring Harbor Symp. Quant. Biol. 38: 655.
Chihara, C., and Fristrom, J., 1973, Develop. Biol. 35: 36.
Chihara, C., Petri, W., Fristrom, J., and King, D., 1972, J. Insect Physiol. 18: 1115.
Cohen, E., Edelman, Emmerich, Fekete, E., Wilhelm and Gilbert, L., 1973, J. Insect Physiol. 19: 1857.
Edelman, I.S., 1975, J. Steroid Biochem. 6: 147.
Emmerich, H., 1970, Z. vergl. Physiol. 68: 385.
Fekete, E., Fristrom, D., Kiss, I., and Fristrom, J., 1975, Wilhelm Roux’ Arch. Entwicklungsmech. Organ. 178: 123.
Fristrom, J., 1972, in “The Biology of Imaginal Discs” (H. Ursprung Nöthiger, eds.) pp. 109–154, Springer-Verlag, Berlin.
Fristrom, J., Logan, W., and Murphy, C., 1973, Develop. Biol. 33: 441.
Fristrom, J., Gregg, T., and Siegel, J., 1974, Develop. Biol. 41: 301.
Kroeger, H., 1963, Nature 200:1234. 1973, Exp. Cell Res. 80: 329.
Lezzi, M., and Gilbert, L., 1972, Gen. Comp. Endocrinol. Suppl. 3: 159.
Logan, W., Fristrom, D., and Fristrom, J., 1975, J. Insect Physiol. 21: 1343.
Mandaron, P., 1973, Develop. Biol. 31: 101.
Milner, M., and Sang, J., 1974, Cell 3: 141.
Nishiura, J., and Fristrom, J., 1975, Proc. Nat. Acad. Sci., USA 72: 2984.
Poodry, C., and Schneiderman, H., 1971, Wilhelm Roux’ Arch. Entwicklungsmech. Organ. 168: 1.
Robb, J., 1969, J. Cell Biol. 41: 876.
Siegel, J., and Fristrom, J., 1976, in“Biology of Drosophila,” (M. Ashburner and T. Wright, eds.) v. II, Academic Press, New York, in press.
Yund, M., and Fristrom, J., 1975a, Develop. Biol. 43: 287.
Yund, M., and Fristrom, J., 1975b, Proc. ICN, UCLA Squaw Valley Conf. Develop. Biol., in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1976 Plenum Press, New York
About this chapter
Cite this chapter
Fristrom, J.W., Chihara, C.J., Kelly, L., Nishiura, J.T. (1976). The Effects of Juvenile Hormone on Imaginal Discs of Drosophila In Vitro: The Role of the Inhibition of Protein Synthesis. In: Gilbert, L.I. (eds) The Juvenile Hormones. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7947-8_30
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7947-8_30
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7949-2
Online ISBN: 978-1-4684-7947-8
eBook Packages: Springer Book Archive