Establishment of a Shoot Culture System for Larix Decidua

Abstract

One limiting aspect in the micropropagation of many conifers is the inability to establish a sustained shoot culture based on axillary bud proliferation. In contrast, all successful commercial micropropagation systems of dicotyledonous woody plants, are based on the establishment of such a shoot culture system. Most conifer microculture systems rely on the one time proliferation of adventitious buds from embryonic tissue, with very few species being capable of reliable and sustained axillary bud proliferation from these adventitious buds.