Abstract
Yellow-poplar can be regenerated from tissue culture by somatic embryogenesis. The transformation of embryogenic cultures should allow the production of transgenic yellow-poplar plants. Direct gene transfer methods have been used to introduce the gene encoding ß-glucuronidase (GUS) into protoplasts (polyethyene-glycol mediated uptake, electroporation) and intact cells (microprojectile bombardment) from suspension cultures. In addition to other parameters, the physiological age of the protoplasts was found to be an important factor in the transient expression of GUS. Intact cells, bombarded with DNA-coated tungsten particles, were found to express the marker gene individually and in cell clusters.
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Literature Cited
Fromm, M., Taylor, L.P., and Walbot, V., 1985, Expression of genes transferred into monocot and dicot cells by electroporation. Proc. Natl. Acad. Sci. USA 82: 5824–5828.
Fromm, M.E., Taylor, L.P., and Walbot, V., 1986, Stable transformation of maize after gene transfer by electroporation. Nature 319: 791–793.
Fromm, M., Callis, J., Taylor, L.P., and Walbot, V., 1987, Electroporation of DNA and RNA into plant protoplasts. Methods in Enzymol. 153: 351–366.
Jefferson, R.A., Kavanaugh, T.A., and Bevan, M.W., 1987, GUS fusions: ß-Glucuronidase as a sensitive and versatile fusion marker in higher plants. EMBO 6: 3901–3907.
Klein, T.M., Gradziel, T., Fromm, M.E., and Sanford, J. C., 1988, Factors influencing gene delivery into Zea mays by high velocity microprojectiles. Biotechnology 6: 559–563.
Merkle, S.A., and Sommer, H.E., 1986, Somatic embryogenesis in tissue cultures of Liriodendron tulipifera. Can. J. For. Res. 16: 420–422.
Merkle, S.A., and Sommer, H.E., 1987, Regeneration of Liriodendron tulipifera (Family Magnoliaceae) from protoplast culture. Amer. J. Bot. 74: 1317–1321.
Meyer, P., Walgenbach, E., Bussmann, K., Hombrecher, G., and Saedler, H., 1985, Synchronized tobacco protoplasts are efficiently transformed by DNA. Mol. Gen. Genet. 201: 513–518.
Okada, K., Takebe, I., and Nagata, T., 1986, Expression and integration of genes introduced into highly synchronized plant protoplasts. Mol. Gen. Genet. 205: 398–403.
Sanford, J.C., 1988, The biolistic process. Trends in Biotech. 6: 299–302.
Toriyama, K., Arimoto, Y., Uchimaya, H., and Hinata, K., 1988, Transgenic rice plants after direct gene transfer into protoplasts. Biotechnology 6: 1072–1075.
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© 1991 Plenum Press, New York
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Wilde, H.D., Meagher, R.B., Merkle, S.A. (1991). Transfer of Foreign Genes into Yellow-Poplar (Liriodendron Tulipifera) . In: Ahuja, M.R. (eds) Woody Plant Biotechnology. NATO ASI Series, vol 210. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7932-4_24
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DOI: https://doi.org/10.1007/978-1-4684-7932-4_24
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4684-7934-8
Online ISBN: 978-1-4684-7932-4
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