Abstract
The recent successes in genetically-engineering trees have in large part been due to advances in both the microculture and the gene transfer technologies. Besides regeneration, a critical problem with microculture has been developing efficient techniques for selecting cells and tissues that are expressing the inserted foreign genes. One solution has been the use of liquid overlays of the selective agent. In regards to gene transfer technologies, both vector-mediated and direct gene transfer techniques have been successful, particularly with Populus. However, the versatility and efficiency of particle bombardment appears to be a major advantage that may lead to the rapid adoption of such methods in biotechnology programs utilizing tree species.
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References
Bekkaoui, F., Pilon, M., Laine, E., Raju, D.S.S., Crosby, W.L., and Dunstan, D.I. 1988. Transient gene expression in electroporated Picea glauca protoplasts. Plant Cell Reports 7:481–484.
Fillatti, J.J., Sellmer, J., McCown, B., Haissig, B. and Cornai, L. 1987. Agrobacterium mediated transformation and regeneration of poplar. Mol. Gen. Genet. 206:192–199.
McCabe, D.E., Swain, W.F., Martinell, B.J. and Christou, P. 1988. Stable transformation of soybean (Glycine max) by particle acceleration. Biotechnology 6:923–926.
McCown, B.H., McCabe, D.E., Russell, D.R., Robison, D.J., Barton, K.Ä., and Raffa, K.F. IN PRESS. Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration. Plant Cell Reports.
McGranahan, G., Leslie, C., Uratsu, S., Martin, L. and Dandekar, A. 1988. Agrobacterium-mediated transformation of walnut somatic embryos and regeneration of transgenic plants. Bio/Technology 6:800–804.
Pythoud, F., Sinkar, V.P., Nester, E.W. and Gordon, M.P. 1987. Increased virulence of Agrobacterium rhizogenes conferred by the vir region of pTiBo542: application to genetic engineering of poplar. Bio/Technology 5:1323–1327.
Seguin, A. and Lalonde, M. 1988. Gene transfer by electroporation in betulaceae protoplasts: Alnus incana. Plant Cell Rep. 7:367–370.
Sellmer, J.C. and McCown, B. H. 1989. Transformation in Populus spp. In: Y.P.S. Bajaj (ed), Plant Protoplasts and Genetic Engineering II. Springer Verlag, Berlin. pp. 155–172.
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© 1991 Plenum Press, New York
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McCown, B.H. (1991). Genetic Transformation of Trees via Direct Gene Transfer. In: Ahuja, M.R. (eds) Woody Plant Biotechnology. NATO ASI Series, vol 210. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7932-4_22
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DOI: https://doi.org/10.1007/978-1-4684-7932-4_22
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