Abstract
Previously we reported the volatilization of intact DNA molecules as large as ~ 410,000 Da by pulsed laser ablation of frozen aqueous solutions deposited on a copper substrate [1]. The laser wavelength was 581 nm and the pulse length was 20 ns (full width). More recently, we have reported volatilization of DNA molecules up to ~ 6 MDa molecular weight [2], and the detection of molecular ions of DNA up to ~ 18,500 Da using a time of flight mass spectrometer [2,3]. The efficiency of ionization was found to increase when the laser was tuned to either 589 or 578 nm. In this paper we assemble information from these two studies, together with additional observations, in order to develop some understanding of the ablation (and ionization) mechanisms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R.W. Nelson, M.J. Rainbow, D.E. Lohr and P. Williams, Science 246 (1989) 1585
P. Williams and R.W. Nelson, Proc. 38th ASMS Conf. on Mass Spectrometry, (1990) 22
R.W. Nelson, R.M. Thomas and P. Williams, Rapid. Commun. Mass Spectrometry 4 (1990) 348
R.W. Nelson, Thesis, Arizona State University, 1990. Unpublished.
J.F. Ready in “Effects of High-power Laser Radiation”, Academic Press, New York (1971) p.70
F. Kreith, “Principles of Heat Transfer”, Harper & Row, New York (1986)
V.P. Skripov, “Metastable Liquids”, Wiley, Chichester (1974)
A.J. Campillo, Naval Research Laboratory, Private Communication.
M.H. Rice and J.M. Walsh, J. Chem. Phys. 26 (1957) 824
E. Becker in “Gas Dynamics”, Academic Press, New York (1968) p.78
R. Kelly and R.W. Dreyfus, Nucl. Instr. Methods Phys. Res. B, 32 (1988) 341
E. Schmidt, “Properties of Water and Steam in SI Units”, Springer-Verlag, Berlin (1981)
J.B. Fenn in “Applied Atomic Collision Physics, Vol. 5”, Academic Press (1982) p. 349
H. Pang and E. Yeung, Anal Chem. 61 (1989) 2546
C.J. Mclean, J.H. Marsh, A.P. Land, A. Clark, R. Jennings, K.W.D. Ledingham, P.T. McCombs, A. Marshall, R.P. Singhal and M. Towrie, Int. J. Mass Spectrom. Ion Processes 96 (1990) 61
F. Hillenkamp, M. Karas, U. Bahr and A. Ingendoh in “Ion Formation from Organic Solids, IFOS V”, ed. A. Hedin, B.U.R. Sundqvist and A. Benninghoven, Wiley, Chichester (1990), p.111.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Williams, P., Nelson, R.W. (1991). On the Mechanism of Volatilization of Large Biomolecules by Pulsed Laser Ablation of Frozen Aqueous Solutions. In: Standing, K.G., Ens, W. (eds) Methods and Mechanisms for Producing Ions from Large Molecules. NATO ASI Series, vol 269. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7926-3_32
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7926-3_32
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7928-7
Online ISBN: 978-1-4684-7926-3
eBook Packages: Springer Book Archive