Some Structural and Functional Properties of Hemoglobin-Containing Liposomes (Hemosomes), A Potential Red Blood Cell Substitute

  • J. Szebeni
  • E. E. Di Iorio
  • H. Hauser
  • K. H. Winterhalter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 238)


There is a growing interest in the encapsulation of hemoglobin (Hb) in liposomes for two reasons: (i) the resulting hemosomes have a potential use as a non-toxic, non-immunogenic red blood cell (RBC) surrogate (1–5), and (ii) they provide a useful RBC model for studying the interaction of Hb with lipid bilayers (6,7). We prepared hemosomes by dispersing various (phospho)lipids in concentrated human RBC lysate, and report here on (i) some morphological characteristics of the resulting particles; (ii) the effect of lipid composition on the amount of entrapped Hb; (iii) the kinetics of CO-binding by entrapped Hb; and (iv) the stability of the lipid membrane and of Hb in hemosomes. To shed light on the molecular mechanism of Hb-liposome interactions, the changes in the intrinsic fluorescence of Hb upon addition to small unilamellar vesicles (SUV) were also analyzed.


Lipid Bilayer Lipid Composition Intrinsic Fluorescence Sodium Cholate Small Unilamellar Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



dipalmitoylphosphatidic acid




egg phosphatidic acid


egg phosphatidylcholine




bovine brain phosphatidylcholine


red blood cell


small unilamellar vesicles


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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • J. Szebeni
    • 1
    • 2
  • E. E. Di Iorio
    • 1
  • H. Hauser
    • 1
  • K. H. Winterhalter
    • 1
  1. 1.Laboratorium fur Biochemie, Eidgenossische Technische HochschuleETH-ZentrumZurichSwitzerland
  2. 2.Department of PhysiologyNational Institute of Food Hygiene and NutritionBudapestHungary

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