Abstract
Werner’s syndrome (WS) has segmental progeroid features and pedigree analysis suggests its autosomal recessive inheritance (Epstein et al., 1966). Two of the clinical characteristics of Werner’s syndrome are hyperkeratinized skin and an excessive excretion of hyaluronic acid into the urine (Tokuraga et al., 1975, Goto and Murata, 1978) suggesting some disorder(s) of connective-tissue metabolism. Cultured fibroblasts from human skin have been used for characterization of genetic defects in many inherited diseases. Epstein et al. (1966) cultured fibroblast-like cells from the skin of patients with WS and noted that they grew very poorly. Since then, WS fibroblasts have been used to find the explanation for the shortened lifespan in vivo and in vitro (Martin et al., 1970). However, the primary molecular defect that retards cell growth has remained elusive. Recently, Salk (1982) published an extensive review article summarizing 15 years of research since that of Epstein et al.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aizawa, S., and Mitsui, Y. (1979), A new cell surface marker of aging in human diploid fibroblasts. J. Cell. Physiol. 100: 383.
Aizawa, S., Mitsui, Y., Kurimoto, F., and Nomura, K. (1980a), Cell surface changes accompanying aging in human diploid fibroblasts. V. role of large major cell surface protein and surface negative charge in aging and transformation associated changes in Con-A mediated red blood cell adsorption. Exp. Cell Res. 127: 143.
Aizawa, S., Mitsui, Y., Kurimoto, F. and Matuoka, K. (1980b), Cell surface changes accompanying aging in human diploid fibroblasts: Effects of tissue, donor age and genotype. Mech. Age. Dev. 13: 297.
Aizawa, S., and Mitsui, Y. (1982), Cell surface and clonal proliferative properties of aging human diploid fibroblasts. Exp. Cell Res. 139: 416.
Beadle, G.F., Mackay, I.R., Whittingham, S., Taggert, G., Harris, A.W., and Harrison, L.C. (1978), Werner’s syndrome, a model of premature aging J. Med. 9: 377.
Dulbecco, R., and Stoker, M.G.P. (1970), Conditions determining initiation of DNA synthesis in 3T3 cells. Proc. Natl. Acad. Sci. ( USA ), 66: 204.
Epstein, C.J., Martin, G.M., Schultz, A.L., and Motulsky, A.G. (1966), Werner’s syndrome: A review of its symptomatology, natural history, pathologic features, genetics and relationship to the natural aging process. Medicine 45: 177.
Fujiwara, Y., Higashikawa, T., and Tatsumi, M. (1977), A retarded rate of DNA replication and normal level of DNA repair in Werner’s syndrome fibroblasts in culture. J. Cell. Physiol. 92: 365.
Goldstein, S., and Singal, D.P. (1974), Alternation of fibroblast gene products in vitro from a subject with Werner’s syndrome. Nature 251: 719.
Goldstein, S., and Niewiarowski, S. (1976), Increased procoagulant activity in cultured fibroblasts from progeria and Werner syndromes of premature aging. Nature 260: 711.
Goto, M., and Murata, K. (1978), Urinary excretion of macro-molecular acidic glycosaminoglycans in Werner’s syndrome. Clin. Chim. Acta. 85: 101.
Martin, G.M., Sprague, C.A., and Epstein, C.J. (1970), Replicative life span of cultivated human cells: Effects of donor’s age, tissue and genotype. Lab. Invest. 23: 86.
Matsumura, T., Mitsui, Y., Fujiwara, Y., Ishii, T., and Shimada, H. (1980), Cell, tissue and organ banks in Japan with special references to the study of premature aging. Jpn. J. Exp. Med. 50: 321.
Matuoka, K., and Mitsui, Y. (1981a), Changes in cell surface glycosaminoglycans in human diploid fibroblasts during in vitro aging. Mech. Age. Devel. 15: 153.
Matuoka, K., and Mitsui, Y. (1981b), Involvement of cell surface heparan sulfate in the density-dependent inhibition of cell proliferation. Cell. Struc. Func. 6: 23.
Mitsui, Y., Aizawa, S., and Matuoka, K. (1979), The relation of cell nuclei and surface membranes to the capacity of cell proliferation in human diploid fibroblasts. In Recent Advances in Gerontology, ed. by Orion, H., Shimada, K., Iriki, M., Maeda, D., Excepta Medica, Amsterdam, 108.
Mitsui, Y., Sakagami, H, Murota, S, and Yamada, M. (1980a), Age related decline in H1 histone fraction in human diploid fibroblast cultures. Exp. Cell. Res. 126: 289.
Mitsui, Y., Matuoka, K., Aizawa, S., and Noda, K. (1980b), New approaches to the characterization of aging human diploid fibroblasts at individual cell level. Adv. Exp. Med. Biol. 129: 5.
Mitsui, Y., and Schneider, E.L. (1976a), Relationship between cell replication and volume in senescent human diploid fibroblasts. Mech. Age. Dev. 5: 45.
Mitsui, Y. and Schneider, E.L. (1976b), Increased nuclear sizes in senescent human diploid fibroblast cultures. Exp. Cell. Res. 100: 147.
Mitsui, Y., and Schneider, E.L. (1976c), Characterization of fractionated human diploid fibroblast cell populations. Exp. Cell. Res. 103: 23.
Mitsui, Y., Smith, J.R., and Schneider, E.R. (1981), Equivalent proliferation potential of different size classes of human diploid fibroblasts. J. Gerontology 36: 416.
Mitsui, Y., and Sakagami, H. (1983), Histone H1 depletion with human cellular aging. Acta Histochem. Cytochem.
Nakao, Y., Kishihara, M., Yoshimi, H., Inoue, Y., Tanaka, K., Sakamoto, N., Matsukura, S., Imura, H., Ichihashi, M. and Fujiwara, Y. (1978), Werner’s syndrome: In vivo and in vitro characteristics as a model of aging. Am. J. Med. 65: 919.
Ohashi, M., Aizawa, S., Ooka, H., Oosawa, H., Kaji, K., Kondo, H., Kobayashi, T., Noumura, Y., Matsuo, M., Mitsui, Y., Murota, S., Yamamoto, K., Ito, H., Shimada, H. and Utakoji, T. (1980), A new human diploid cell strain, TIG-1, for the research on cellular aging. Exp. Geront. 15: 121.
Salk, D. (1982), Werner’s syndrome: A review of recent research with analysis of connective tissue metabolism, growth control of cultured cells and chromosomal aberrations. Hum. Genet. in press.
Schneider, E.L., and Mitsui, Y. (1976), Relationship between in vitro cellular aging and in vivo human age. Proc. Natl. Acad. Sci. ( USA ) 73: 3584.
Schneider, E.L., and Mitsui, Y. (1978), The effect of serum batch on the in vitro life spans of cell cultures derived from old and young human donors. Exp. Cell. Res. 115: 47.
Sluke, G., Schachtschabel, D.O., and Werver, J. (1981), Age-related changes in the distribution pattern of glycosaminoglycans synthesized by cultured human diploid fibroblasts (WI-38). Mech. Age. Devel. 16: 19.
Smith, J.C., Singh, J.P, Lillquist, J.S., Goon, D.S. and Stiles, C.D. (1982), Growth factors adherent to cell substrate are mitogenically active in situ. Nature 296: 154.
Tajima, T., Watanabe, T., Iijima, K., Ohshika, Y. and Yamaguchi, H. (1981), The increase of glycosaminoglycans synthesis and accumulation on the cell surface of cultured skin fibroblasts in Werner’s syndrome. Exp. Path. 20: 221.
Takeuchi, F., Hanaoka, F., Goto, M., Akaoka, I., Hori, T., Yamada, M., and Miyamoto, T. (1982), Altered frequency of initiation sites of DNA replication in Werner’s syndrome cells. Hum. Genet. 60: 365.
Tokunaga, M., Futami, T., Wakamatsu, E., Endo, M. and Yoshizawa, Z. (1975), Werner’s syndrome as “hyaluronuria”, Clinica Chim. Acta. 62: 89.
Wever, J., Schachtshabel, D.O., Sluke, G. and Wever, G. (1980), Effect of short-or long-term treatment with exogenous glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture. Mech. Age. Devel. 14: 89.
Yamamoto, K., Yamamoto, M., and Ooka, H. (1977), Cell surface changes associated with aging of chick embryo fibroblasts in culture. Exp. Cell. Res. 108: 87
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Plenum Press, New York
About this chapter
Cite this chapter
Mitsui, Y., Yamamoto, K., Yamamoto, M., Matuoka, K. (1985). Cell Surface Changes in Senescent and Werner’s Syndrome Fibroblasts: Their Role in Cell Proliferation. In: Salk, D., Fujiwara, Y., Martin, G.M. (eds) Werner’s Syndrome and Human Aging. Advances in Experimental Medicine and Biology, vol 190. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7853-2_30
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7853-2_30
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7855-6
Online ISBN: 978-1-4684-7853-2
eBook Packages: Springer Book Archive