Summary
The renal epoxygenase has been demonstrated to be an active pathway for the conversion of arachidonic acid (AA) to 11,12-epoxide, 19-hydroxy and 20-hydroxy-eicosatetraenoic acids. The epoxygenase activity was found to be located predominantly in the renal cortex. This renal epoxygenase pathway also transformed PGI2 to a new, previously unreported, metabolite. This metabolite was isolated and identified by radio gas chromatography and gas chromatography-mass spectrometry as 5-hydroxy-6-keto-PGFlα. Its structure was further confirmed by comparison of the mass-spectra to that of the synthetic standard. The formation of 5-hydroxy-6-keto-PGF1α in the kidney suggested epoxidation of prostacyclin via the renal epoxygenase as an alternative pathway of PGI2 metabolism.
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© 1985 Plenum Press, New York
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Wong, P.YK., McGiff, J.C., Schwartzman, M., Sun, F.F. (1985). Epoxidation of Arachidonic Acid and Prostacyclin in the Kidney. In: Samuelsson, B., Berti, F., Folco, G.C., Velo, G.P. (eds) Drugs Affecting Leukotrienes and Other Eicosanoid Pathways. NATO ASI Series, vol 95. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7841-9_16
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DOI: https://doi.org/10.1007/978-1-4684-7841-9_16
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