Capillary Supply and Utilization of Intracellular Oxygen in the Left Ventricular Myocardium from Rats Adapted to High Altitude

  • J. Moravec
  • F. Cluzeaud
  • K. Rakusan
  • Z. Turek
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 159)


The chronical exposure of young rats to a simulated altitude of 3500 m results in number of functional adaptations (Grandtner et al., 1974; Turek et al., 1972a). As concerns the heart, a multiplication of capillaries have been noted under chronical hypoxia (Turek, 1972b); Friedman et al., 1973). The stay in the hypoxic chamber leads also to an increase of the heart weight (heart hypertrophy). However, the extent of the latter can vary considerably: in rats born and rised in the hypoxic conditions (“natives”) a bilateral cardiac hypertrophy regularly occurs (Turek et al., 1972b). In those born at sea level and exposed to the same hypoxia fran their youth only (“newcaners”), the increase of the heart weight is restricted exclusively to the right ventricle (Grandtner et al., 1974). In addition, the hearts of the above two groups differ from functional point of view. While in the “natives” the cardiac output is 50 per cent depressed, it is elevated in the “newcaners” (Turek et al., 1972a). The amount of kinetic work supplied by 1 g of the left ventricular myocardium is therefore significantly higher in hearts of the latter animals. This elevated work output of the heart is apparently enabled by higher rates of the oxidative energy production. An increase in the glycolytic fluxes (Hochachka et al., 1977) as well as the accumulation of respiratory enzymes in heart homogenate (Shertzer et al., 1972; Tappan et al., 1957) may underlie such an adaptation to chronical hypoxia. In this work we tried to assess the kinetic aspects of mitochondrial function using the optical methods developed by Chance (Chance, 1976; Sugano et al., 1974). The hearts from rats exposed to the early (“natives”) and to the late (“newcomers”) hypoxia were compared. The left ventricular vascularization was also studied in these two groups by a new morphanetric approach (Rakusan et al., 1980) derived from the work of Loats et co-workers (Coats et al., 1978).


Diffusion Distance Chronical Hypoxia Heart Weight Pyridine Nucleotide Left Ventricular Myocardium 
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  1. 1.
    Antonini, E., Brunori, M., Colosimo, A., Greenwood, C., Wilson, T. M.: Oxygen pulsed cytochrome c oxidase: functional properties and catalytic relevance. Proc. Natl. Acad. Sci., 74: 3128–3131, 1977.PubMedCrossRefGoogle Scholar
  2. 2.
    Chance, B. Pyridine nucleotide as an indicator of the oxygen requirements for energy-linked functions of the mitochondria. Circ. Res., 38 (suppl. 1): 31–38, 1976.Google Scholar
  3. 3.
    Coburn, R. F. Ploemakers, F., Gondrie, P., Abboud, R.: Myocardial myoglobin oxygen tension. Am. J. Physiol., 224: 870–878, 1973.PubMedGoogle Scholar
  4. 4.
    Friedman, I., Moravec, J., Reichart, E., Hatt, P. Y.: Subacute myocardial hypoxia in the rat. An E. M. Study of the left ventricular myocardium. J. Mol. Cell. Cardiol., 5: 125–132, 1973.Google Scholar
  5. 5.
    Grandtner, M., Turek, Z., Kreuzer, F.: Cardiac hypertrophy in the first generation of rats native to simulated high altitude. Pflugers Arch., 350: 241–248, 1974.PubMedCrossRefGoogle Scholar
  6. 6.
    Hochachka, P. V., Guppy, M.: Variations on a the by Embden, Meyerlof and Parnas. In: Oxygen & Physiological Function, Jobsis, F. F. ed., Professional Information Library, Dallas, 1977, pp. 292–310.Google Scholar
  7. 7.
    Loats, J. T., Sillau, A. H., Banchero, N.: How to quantify skeletal muscle capillarity. In: Oxygen Transport to Tissue, Vol. 3. Silver, I. S. Erecinska, M., Bicher, H. I., eds., Plenum Press, New York, 1978, pp. 41–48.Google Scholar
  8. 8.
    Libbers, D. W.: Measuring methods for the analysis of tissue oxygen supply. In: Oxygen and Physiological Function. Jobsis, F. F. ed., Professional Information Library, Dallas, 1977, pp. 62–71.Google Scholar
  9. 9.
    Mela, L., Goodwin, C. W., Miller, L. D.: In vivo control of mitochondrial enzyme concentrations and activity by oxygen. Am. J. Physiol., 231, 1811–1816, 1976.PubMedGoogle Scholar
  10. 10.
    Moravec, J., Moravec, M., Hatt, P. Y.: Rate of pyridine nucleotide oxidation and cytochrcane oxidase interaction with intracellular oxygen in hearts from rats with compensated volume overload. Pflugers Arch. (in apress).Google Scholar
  11. 11.
    Rakusan, K., Moravec, J., Hatt, P. Y.: Regional capillary supply in the normal and hypertrophied heart. Microvasc. Res., 20: 319–326, 1980.Google Scholar
  12. 12.
    Shertzer, H.G., Cascarano, J.: Mitochondrial alterations in heart, liver and kidney of altitude-acclimated rats. Am. J. Physiol., 223: 632–636, 1972.PubMedGoogle Scholar
  13. 13.
    Sugano, T., Ohnish, N., Chance, B.: Mitochondrial function under hypoxic conditions. The states of cytochran c reduction and of energy metabolism. Biochim. Biophys. Acta, 347, 340–358, 1974.Google Scholar
  14. 14.
    Tamura, M., Oshino, N., Chance, B.: The myoglobin probed optical studies of myocardial energy metabolism. In: Oxygen Transport to Tissue. Vol. 3. Silver, I. S., Erecinska, M., Bicher, H. I., eds., Plenum Press, New York, 1978Google Scholar
  15. 15.
    Tappan, D. V., Reynafarje, B. D., Potter, R., Hurtado, A.: Alterations in enzymes and metabolites resulting f adaptation to low oxygen tensions. Am. J. Physiol., 190: 93–98, 1957.PubMedGoogle Scholar
  16. 16.
    Turek, Z., Ringnalda, B. E. M., Hoofd, L. J. D., Frans, A., Kreuzer, F.: Cardiac output, arterial and mixed venous 02 saturation and blood dissociation curbe in growing rats adapted to a simulated altitude. Pflugers Arch., 335: 10–18, 1972.PubMedCrossRefGoogle Scholar
  17. 17.
    Turek, Z., Grandtner, M., Kreuzer, F.: Cardiac hypertrophy, capillary and muscle fiber density, muscle fiber diameter, capillary radius and diffusion distance in the myocardium of growing rats adapted to a simulated altitude. Pflugers Arch., 335: 19–28, 1972.PubMedCrossRefGoogle Scholar
  18. 18.
    Turek, Z., Ringnalda, B. E. M., Grandtner, M., Kreuzer, F.: Myoglobin distribution in the heart of growing rats exposed to a simulated altitude. Pflugers Arch., 340: 1–10, 1973.PubMedCrossRefGoogle Scholar
  19. 19.
    Turek, Z., Maischeider, M., Claessens, R. A., Rinnalda, B. E. M., Kreuzer, F.: Coronary blood flow in rats native to simulated high altitude and in rats exposed to it later in life. Pflugers Arch., 355: 49–62, 1975.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. Moravec
    • 1
    • 2
  • F. Cluzeaud
    • 1
    • 2
  • K. Rakusan
    • 1
    • 2
  • Z. Turek
    • 1
    • 2
  1. 1.Hopital Leon BernardI.N.S.E.R.M. U2Limeil-BrevannesFrance
  2. 2.Department of PhysiologyUniversity of NijmegenNijmegenThe Netherlands

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