Abstract
Prostanoic derivatives, as prostaglandin, thromboxane, prostacyclin and their metabolites, are generally present in biological media at very low concentrations (10−9–10−11 M). Therefore assays with high sensitivity often are required for their measurement. Two techniques are widely used: mass spectrometry combined with gas chromatography (GS-MS), which is particularly specifics1 and radio-immunoassay (RIA) which is particularly sensitive and convenient for large series2,7. In our laboratory, immunological methods have been developed using radioactive8 or non-radioactive tracers5,9; iodinated derivatives (as PG−125I-Histamine) have been shown to be more sensitive convenient 10–14, In this paper some aspects of the use of iodinated tracers will be presented and a new strategy for increasing the specificity of RIA of prostanoic derivatives will be proposed.
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Dray, F. (1982). Sensitivity and Specificity of Prostanoic Derivatives Radioimmunoassays: New Strategy. In: Swillens, S., Dumont, J.E. (eds) Cell Regulation by Intracellular Signals. NATO Advanced Study Institutes Series, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7718-4_12
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DOI: https://doi.org/10.1007/978-1-4684-7718-4_12
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