Abstract
Animal cells in culture, in marked contrast to microorganisms (Chapters 10–12), are able to grow on only a limited number of carbohydrates other than glucose. The early literature contains a number of reports on the ability of carbohydrates to support growth of various cell types (Eagle et al., 1958; Morgan and Morton, 1960) but some investigations are open to the criticism that they did not take fully into account the facts that many commercially available carbohydrates are contaminated with glucose, that serum itself contains glucose, and that the cells may thus really have been utilizing glucose.
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References
Aithal, H. N., Walsh-Reitz, M. M., and Toback, F. G., 1983, Appearance of a cytosolic protein that stimulates glyceraldehyde-3-phosphate dehydrogenase activity during initiation of renal epithelial cell growth, Proc. Natl. Acad. Sci. USA 80: 2941–2945.
Amos, H., Leventhal, M., Chu, L., and Karnovsky, M. J., 1976, Modifications of mammalian cell surfaces induced by sugars: Scanning electron microscopy, Cell 7: 97–103.
Ardawi, M. S. M., and Newsholme, E. A., 1982, Maximum activities of some enzymes of glycolysis, the tricarboxylic acid cycle and ketone-body and glutamine utilisation pathways in lymphocytes of the rat, Biochem. J. 208: 743–748.
Arinze, I. J., Raghunathan, R., and Russell, J. D., 1978, Induction of mitochondrial phosphoenolpyruvate carboxykinase in cultured human fibroblasts, Biochim. Biophys. Acta 531: 792–804.
Attenello, J. W., and Lee, A. S., 1984, Regulation of a hybrid gene by glucose and temperature in hamster fibroblasts, Science 226: 187–190.
Avner, P., Dubois, P., Nicolas, J. F., Jakob, H., Gaillard, J., and Jacob, F., 1977, Mouse teratocarcinoma: Carbon source utilisation patterns for growth and in vitro differentiation, Exp. Cell Res. 105: 39–50.
Bailey, J. M., Gey, G. O., and Gey, M. K., 1959, The carbohydrate nutrition and metabolism of a strain of mammalian cells (MB III strain of mouse lymphoblasts) growing in vitro, J. Biol. Chem. 234: 1042–1047.
Benn, P. A., Kelley, R. I., Mellman, W. J., Amer, L., Boches, F. S., Markus, H. B., Nichols, W., and Hoffman, B., 1981, Reversion from deficiency of galactose 1-phosphate uridyltransferase (GALT) in an SV40-transformed human fibroblast line, Somat. Cell Genet. 7: 667–682.
Bertolotti, R., 1977a, A selective system for hepatoma cells producing gluconeogenic enzymes, Somat. Cell Genet. 3: 365–380.
Bertolotti, R., 1977b, Expression of differentiated functions in hepatoma cell hybrids: Selection in glucose-free media of segregated hybrid cells which re-express gluconeogenic enzymes, Somat. Cell Genet. 3: 579–602.
Bishayee, S., and Das, M., 1981, Aberrant energy metabolism in a variant epidermal growth factor receptor-negative fibroblastic cell line, FEBS Lett. 127: 237–240.
Bloch, R., Betschart, B., and Burger, M. M., 1977, Cell culture in serum depleted of glycosidases by heating, Exp. Cell Res. 104: 143–152.
Blomquist, C. H., Gregg, C. T., and Tobey, R. A., 1971, Enzyme and co-enzyme levels, oxygen uptake and lactate production in synchronised cultures of Chinese hamster cells, Exp. Cell Res. 66: 75–80.
Bruni, P., Faranraro, M., Vasta, V., and D’Alessandro, A., 1983, Increase of the glycolytic rate in human resting fibroblasts following serum stimulation: The possible role of the fructose 2,6bisphosphate, FEBS Lett. 159: 39–42.
Burns, R. L., Rossenberger, P. G., and Klebe, R. J., 1976, Carbohydrate preferences of mammalian cells, J. Cell. Physiol. 88: 307–316.
Bustamante, E., and Pedersen, P. L., 1977, High aerobic glycolysis of rat hepatoma cells in culture: Role of mitochondrial hexokinase, Proc. Natl. Acad. Sci. USA 74: 3735–3739.
Bustamante, E., Morris, H. P., and Pedersen, P. L., 1981, Energy metabolism of tumour cells: Requirement for a hexokinase with a propensity for mitochondrial binding, J. Biol. Chem. 256: 8699–8704.
Cassio, D., 1984, Re-expression of hepatic functions in mouse hepatoma x rat hepatoma hybrids, Differentiation 26: 77–82.
Chen, Y. T., Mattison, D. R., Feigenbaum, L., Fukui, H., and Schulman, J. D., 1981, Reduction in oocyte number following prenatal exposure to a diet high in galactose, Science 214: 1145–1147.
Clayton, D. F., and Darnell, J. E., 1983, Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes, Mol. Cell. Biol. 3: 1552–1561.
Cogoli, A., Tschopp, A., and Fuchs-Bislin, P., 1984, Cell sensitivity to gravity, Science 225: 228–230.
Colby, C., and Romano, A. H., 1974, Phosphorylation but not transport of sugars is enhanced in virus-transformed mouse 3T3 cells, J. Cell. Physiol. 85: 15–24.
Cooper, J. A., Reiss, N. A., Schwartz, R. J., and Hunter, T., 1983, Three glycolytic enzymes are phosphorylated at tyrosine in cells transformed by Rous sarcoma virus, Nature 302: 218–223.
Cox, R. P., and Gesner, B. M., 1965, Effect of simple sugars on the morphology and growth pattern of mammalian cell cultures, Proc. Natl. Acad. Sci. USA 54: 1571–1579.
Dahl, R. H., and Morse, M. L., 1979, Differential metabolism of mannose by Chinese hamster cell lines, Exp. Cell Res. 121: 277–282.
Dahl, R. H., Morrissey, A., Puck, T. T., and Morse, M. L., 1976, Carbohydrate energy sources for Chinese hamster cells in culture, Proc. Soc. Exp. Biol. Med. 153: 251–253.
Demetrakopoulos, G. E., and Amos, H., 1976, D-Xylose and xylitol: Previously unrecognised sole carbon and energy sources for chick and mammalian cells, Biochem. Biophys. Res. Commun. 72: 1169–1176.
Demetrakopoulos, G. E., Gonzalez, F., Colofiore, J., and Amos, H., 1977, Growth of chick and mammalian cells on D-xylose, Exp. Cell Res. 106: 167–173.
Deschatrette, J., and Weiss, M. C., 1974, Characterisation of differentiated and dedifferentiated clones from a rat hepatoma, Biochimie 56: 1603–1611.
Deschatrette, J., Moore, E. E., Dubois, M., and Weiss, M. C., 1980, Dedifferentiated variants of a rat hepatoma: Reversion analysis, Cell 19: 1043–1051.
Diamond, I., Legg, A., Schneider, J. A., and Rozengurt, E., 1978, Glycolysis in quiescent cultures of 3T3 cells: Stimulation by serum, epidermal growth factor, and insulin in intact cells and persistence of the stimulation after cell homogenization, J. Biol. Chem. 253: 866–871.
D’Urso, M., Mareni, C., Toniolo, D., Piscopo, M., Schlessinger, D., and Luzzatto, L., 1983, Regulation of glucose 6-phosphate dehydrogenase expression in CHO—human fibroblast somatic cell hybrids, Somat. Cell Genet. 9: 429–443.
Eagle, H., Barban, S., Levy, M., and Schulze, H. O., 1958, The utilisation of carbohydrates by human cell cultures, J. Biol. Chem. 233: 551–558.
Emerman, J. T., Bartley, J. C., and Bissell, M. J., 1981, Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells, Exp. Cell Res. 134: 241–250.
Faik, P., and Morgan, M. J., 1976, Carbohydrate metabolism in Chinese hamster cells, Biochem. Soc. Trans. 4: 1043–1045.
Faik, P., and Morgan, M. J., 1977a, A method of isolation of Chinese hamster cell variants with an altered ability to utilise carbohydrates, Cell Biol. Int. Rep. 1: 555–562.
Faik, P., and Morgan, M. J., 1977b, Properties of carbohydrate utilising variants of Chinese hamster cells, Cell Biol. Int. Rep. 1: 563–570.
Faik, P., and Morgan, M. J., 1980, The regulation of carbohydrate metabolism in animal cells: Isolation of variants able to utilise lactate, Biochem. Soc. Trans. 8: 632–633.
Faik, P., and Morgan, M. J., 1984, Regulation of hexose uptake in Chinese hamster ovary cells, Biochem. Soc. Trans. 12: 10.
Faik, P., Rawson, S., Walker, J. H., and Morgan, M. J., 1986, Introduction of human phosphoglycerate kinase (PGK) cDNA into a PGK-deficient line of Chinese hamster ovary cells, Genet. Res.,in press.
Fodge, D. W., and Rubin, H., 1973, Activation of phosphofructokinase by stimulants of cell multiplication, Nature 246: 181–183.
Fukushima, N., Cohen-Khallas, M., and Kalant, N., 1981, Galactose and glucose metabolism by cultured hepatocytes: Responsiveness to insulin and the effect of age, Dev. Biol. 84: 359–363.
Giovanni, M. Y., Kessel, D., and Gluck, M. C., 1981, Specific monosaccharide inhibition of active sodium channels in neuroblastoma cells, Proc. Natl. Acad. Sci. USA 78: 1250–1254.
Gregory, S. H., and Bose, S. K., 1977, Density-dependent changes in hexose transport, glycolytic enzyme levels and glycolytic rates, in uninfected and murine sarcoma virus-transformed rat kidney cells, Exp. Cell Res. 110: 387–397.
Gregory, S. H., and Bose, S. K., 1979, Glycolytic enzyme activities in malignant cells grown in vitro and in vivo, Cancer Lett. 7: 319–324.
Gunn, J. M., Shinozuka, H., and Williams, G. M., 1975, Enhancement of phenotypic expression in cultured malignant liver epithelial cells by a complex medium, J. Cell. Physiol. 87: 79–89.
Halban, P. A., Praz, G. A., and Wollheim, C. B., 1983, Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F), Biochem. J. 212: 439–443.
Harris, M., and Kutsky, P. B., 1953, Utilisation of added sugars by chick heart fibroblasts in dialysed media, J. Cell. Comp. Physiol. 42: 449–466.
Hers, H. G., and Van Schaftingen, E., 1982, Fructose 2,6-bisphosphate 2 years after its discovery, Biochem. J. 206: 1–12.
Hill, H. Z., 1976, The effect of pH on incorporation of galactose by a normal human cell line and cell lines from patients with defective galactose metabolism, J. Cell. Physiol. 87: 313–320.
Hoffee, P., Jargiello, P., Zaner, L., and Martin, J., 1977, Pentose utilising variants of Novikoff hepatoma cells: Modification of growth and morphological properties, J. Cell. Physiol. 91: 3950.
Hutz, M. H., Michelson, A. M., Antonarakis, S. E., Orkin, S. H., and Kazazian, H. H., 1984, Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome, Hum. Genet. 66: 217–219.
Isaka, T., Yoshida, M., Owada, M., and Toyoshima, K., 1975, Alterations in membrane polypeptides of chick embryo fibroblasts induced by transformation with avian sarcoma viruses, Virology 65: 226–237.
Jargiello, P., 1978, Pentose utilising variants of Novikoff hepatoma cells: Phenotypic characterisation, Somat. Cell Genet. 4: 647–660.
Jargiello, P., 1980, Multiple genetic changes determine ribose utilisation by Novikoff hepatoma cell variants, Biochim. Biophys. Acta 632: 507–516.
Jargiello, P., 1982, Altered expression of ribokinase activity in Novikoff hepatoma variants, Biochim. Biophys. Acta 698: 78–85.
Johnson, G. S., and Schwartz, J. P., 1976, Effects of sugars on the physiology of cultured fibroblasts, Exp. Cell Res. 97: 281–290.
Kajstura, J., and Korohoda, W., 1983, Significance of energy metabolism pathways for stimulation of DNA synthesis by cell migration and serum, Eur. J. Cell Biol. 31: 9–14.
Kielty, C. M., Povey, S., and Hopkinson, D. A., 1981, Regulation of expression of liver-specific enzymes. 1. Detection in mammalian tissues and cultured cells, Ann. Hum. Genet. 45: 341–356.
Kielty, C. M., Povey, S., and Hopkinson, D. A., 1982, Regulation of expression of liver-specific enzymes. 3. Further analysis of a series of rat hepatoma x human somatic cell hybrids, Ann. Hum. Genet. 46: 307–327.
Kletzien, R. F., and Perdue, J. F., 1974, Sugar transport in chick embryo fibroblasts, III. Evidence for host-transcriptional and host-translational regulation of transport following serum addition, J. Biol. Chem. 249: 3383–3387.
Krooth, R. S., and Weinberg, A. N., 1961, Studies on cell lines developed from the tissues of patients with galactosemia, J. Exp. Med. 113: 1155–1171.
Kuchka, M., Markus, H. B., and Mellman, W. J., 1981, Influence of hexose conditions on glutamine oxidation of SV40-transformed and diploid fibroblast human cell lines, Biochem. Med. 26: 356–364.
Landau, B. R., and Wood, H. G., 1983, The pentose cycle in animal tissues: Evidence for the classical and against the `L-type’ pathway, Trends Biochem. Sci. 8: 292–296.
Lanks, K. W., 1983, Metabolite regulation of heat shock protein levels, Proc. Natl. Acad. Sci. USA 80: 5325–5329.
Lazo, P. A., 1981, Amino acids and glucose utilisation by different metabolic pathways in ascites-tumour cells, Eur. J. Biochem. 117: 19–25.
Lee, A. S., 1981, The accumulation of three specific proteins related to glucose-regulated proteins in a temperature-sensitive hamster mutant cell line K12, J. Cell. Physiol. 106: 119–125.
Lee, A. S., Bell, J., and Ting, J., 1984, Biochemical characterization of the 94- and 78-kilodalton glucose-regulated proteins in hamster fibroblasts, J. Biol. Chem. 259: 4616–4621.
Levilliers, J., and Weiss, M. C., 1983, Differentiation is not restored in hybrids between independent variants of a rat hepatoma, Somat. Cell Genet. 9: 407–413.
Lin, A. Y., and Lee, A. S., 1984, Induction of two genes by glucose starvation in hamster fibroblasts, Proc. Natl. Acad. Sci. USA 81: 988–992.
McGowan, J. A., Russell, W. E., and Bucher, L. R., 1984, Hepatocyte DNA replication: Effect of nutrients and intermediary metabolites, Fed. Proc. 43: 131–133.
McKeehan, W. L., 1984, Control of normal and transformed cell proliferation by growth factor—nutrient interactions, Fed. Proc. 43: 113–115.
McKeehan, W. L., McKeehan, K. A., and Calkins, D., 1982, Epidermal growth factor modifies Cat+, Mg2+ and 2-oxocarboxylic acid, but not K+ and phosphate ion requirement for multiplication of human fibroblasts, Exp. Cell Res. 140: 25–30.
Maiti, I. B., Comlan de Souza, A., and Thirion, J. P., 1981, Biochemical and genetic characterization of respiration-deficient mutants of Chinese hamster cells with a Gal phenotype, Somat. Cell Genet. 7: 567–582.
Malaisse, W. J., Malaisse-Lagae, F., Sener, A., Van Schaftingen, E., and Hers, H. G., 1981, Is the glucose-induced stimulation of glycolysis in pancreatic islets attributable to activation of phosphofructokinase by fructose 2,6-bisphosphate?, FEBS Lett. 125: 217–219.
Meglasson, M. D., and Matschinsky, F. M., 1984, New perspectives on pancreatic islet glucokinase, Am. J. Physiol. 246: E1 - E13.
Melnykovych, G. and Bishop, C. F., 1972, Utilisation of hexoses and synthesis of glycogen in two strains of HeLa cells, In Vitro 7: 397–405.
Michelson, A. M., Markham, A. F., and Orkin, S. H., 1983, Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase, Proc. Natl. Acad. Sci. USA 80: 427–476.
Miwa, S., Nakashima, K., Oda, S., Ogawa, H., Nakafuji, H., Arlma, M., Okuna, T., Nakashima, T., 1972, Phosphoglycerate kinase (PGK) deficiency hereditary nonspherocytic hemolytic anemia: Report of a case found in a Japanese family, Acta Haematol. Jpn. 35: 570–574.
Moore, E. E., and Weiss, M. C., 1982, Selective isolation of stable and unstable dedifferentiated variants from a rat hepatoma cell line, J. Cell. Physiol. 111: 1–8.
Morgan, J., and Morton, H., 1960, Carbohydrate utilisation by chick embryonic heart cultures, Can. J. Biochem. Physiol. 35: 69–78.
Morgan, M. J., 1981, The pentose phosphate pathway: Evidence for the indispensable role of glucose-phosphate isomerase, FEBS Lett. 130: 124–126.
Morgan, M. J., and Faik, P., 1980, The regulation of carbohydrate metabolism in animal cells: Isolation of a glycolytic variant of Chinese hamster ovary cells, Cell Biol. Int. Rep. 4: 121–127.
Morgan, M. J., and Faik, P., 1981, Carbohydrate metabolism in cultured animal cells, Biosci. Rep. 1: 669–686.
Morgan, M. J., Faik, P., and Walker, S. W., 1980, The regulation of carbohydrate metabolism in animal cells: Isolation of a glycolytic variant, Biochem. Soc. Trans. 8: 631–632.
Morgan, M. J., Bowness, K. M., and Faik, P., 1981, Regulation of carbohydrate metabolism in cultured mammalian cells: Energy provision in a glycolytic mutant, Biosci. Rep. 1: 811–817.
Morgan, M. J., Bowness, K. M., and Faik, P., 1983a, Energy provision in Chinese hamster ovary cells, Biochem. Soc. Trans. 11: 725–726.
Morgan, M. J., Faik, P., and Calvert, J., 1983b, Genetics of carbohydrate metabolism in animal cells, Genet. Res. 41: 307.
Nepokroeff, C. M., Lakshmann, M. R., Ness, G. C., Muesing, R. A., Kleinsek, D. A., and Porter, J. W., 1974, Co-ordinate control of rat liver lipogenic enzymes by insulin. Arch. Biochem. Biophys. 162: 340–344.
Ovadi, J., and Keleti, T., 1978, Kinetic evidence for interaction between aldolase and Dglyceraldehyde-3-phosphate dehydrogenase, Eur. J. Biochem. 85: 157–161.
Pauwels, P. J., Opperdoes, F. R., and Trouet, A., 1984, Effect of oxygen and glucose availability on the glycolytic rate in neuroblastoma cells under different conditions of culture, Neurochem. Int. 6: 467–473.
Piechaczyk, M., Blanchard, J. M., Riaad-EI Sabouty, S., Dani, C., Marty, L, and Jeanteur, P., 1984, Unusual abundance of vertebrate 3-phosphate dehydrogenase pseudogenes, Nature 312: 469–471.
Pinto, M., Appay, M. D., Simon-Assman, P., Chevalier, G., Dracopoli, N., Fogh, J., and Zweibaum, A., 1982, Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium, Biol. Cell. 44: 193–196.
Pouysségur, J., Shiu, R. P. C., and Pastan, I., 1977, Induction of two transformation-sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation, Cell 11: 941–947.
Pouysségur, J., Franchi, A., Salomon, J.-C., and Silvestre, P., 1980, Isolation of a Chinese hamster fibroblast mutant defective in hexose transport and aerobic glycolysis: Its use to dissect the malignant phenotype, Proc. Natl. Acad. Sci. USA 77: 2698–2701.
Racker, E., 1976, Why do tumour cells have a high aerobic glycolysis?, J. Cell. Physiol. 89: 697–700.
Racker, E., 1984, Resolution and reconstitution of biological pathways from 1919 to 1984, Fed. Proc. 42: 2899–2909.
Racker, E., Johnson, J. H., and Blackwell, M. D., 1983, The role of ATPase in glycolysis of Ehrlich ascites tumour cells, J. Biol. Chem. 258: 3702–3705.
Reitzer, L. J., Wise, B. M., and Kennel, D., 1980, The pentose cycle: Control and essential function in HeLa cell nucleic acid synthesis, J. Biol. Chem. 255: 5616–5626.
Rheinwald, J. G., and Green, H., 1974, Growth of cultured mammalian cells on secondary glucose sources, Cell 2: 287–293.
Romano, A. H., and Connell, N. D., 1982a, 6-Deoxy-D-glucose and D-xylose, analogs for the study of D-glucose transport by mouse 3T3 cells, J. Cell. Physiol. 111: 77–82.
Romano, A. H., and Connell, N. D., 1982b, Effect of glucose uptake on growth rate of mouse 3T3 cells, J. Cell. Physiol. 111: 195–200.
Rosenstraus, M., and Chasin, L. A., 1975, Isolation of mammalian cell mutants deficient in glucose 6-phosphate dehydrogenase activity: Linkage to hypoxanthine phosphoribosyltransferase, Proc. Natl. Acad. Sci. USA 72: 493–497.
Russell, J. D., and De Mars, R., 1967, UDP glucose: a-n-galactose-l-phosphate uridyl transferase activity in cultured human fibroblasts, Biochem. Genet. 1: 11–24.
Scannell, J., and Morgan, M. J., 1980, The regulation of carbohydrate metabolism in animal cells: Growth on starch and maltose, Biochem. Soc. Trans. 8: 633–634.
Scannell, J., and Morgan, M. J., 1982, The regulation of carbohydrate metabolism in animal cells: Isolation of starch-and maltose-utilising variants, Biosci. Rep. 2: 99–106.
Schneider, J. A., Diamond, I., and Rozengurt, E., 1978, Glycolysis in quiescent cultures of 3T3 cells: Addition of serum, epidermal growth factor, and insulin increases the activity of phosphofructokinase in a protein synthesis-indepedent manner, J. Biol. Chem. 253: 872–877.
Schwartz, J. P., and Johnson, G. S., 1976, Metabolic effects of glucose deprivation and of various sugars in normal and transformed fibroblast cell lines, Arch. Biochem. Biophys. 173: 237–245.
Sens, D. A., Hochstadt, B., and Amos, H., 1982, Effects of pyruvate on the growth of normal and transformed hamster embryo fibroblasts, J. Cell. Physiol. 110: 329–335.
Silnutzer, J., and Jargiello, P., 1981, Extinction and expression of the ribose-positive phenotype in hybrid Novikoff hepatoma cells, Somat. Cell Genet. 7: 119–131.
Singer-Sam, J., Simmer, R. L., Keith, D. M., Shirley, L., Teplitz, M., Itakura, K., Gartler, S. M., and Riggs, A. D., 1983, Isolation of a cDNA clone for human X-linked 3-phosphoglycerate kinase by use of a mixture of synthetic oligodeoxyribonucleotides as a detection probe, Proc. Natl. Acad. Sci. USA 80: 802–806.
Singh, M., Singh, V. N., August, G. T., and Horecker, B. L., 1974a, Alterations in glucose metabolism in chick embryo cells transformed by Rous sarcoma virus: Transformation-specific changes in the activities of key enzymes of the glycolytic and hexose monophosphate shunt pathways, Arch. Biochem. Biophys. 165: 240–246.
Singh, M., Singh, V. N., August, G. T., and Horecker, B. L., 1974b, Alterations in glucose metabolism in chick embryo cells transformed by Rous sarcoma virus: Intracellular levels of glycolytic intermediates, Proc. Nad. Acad. Sci. USA 71: 4129–4132.
Smith, M. L., and Buchanan, J. M., 1979, Nucleotide and pentose synthesis after serum-stimulation of resting 3T6 fibroblasts, J. Cell. Physiol. 101: 293–310.
Sols, A., Cadenas, E., and Alvarado, F., 1960, Enzymatic basis of mannose toxicity in honey bees, Science 131: 297–298.
Stern, E. S., and Krooth, R. S., 1975, Studies on the regulation of the three enzymes of the Leloir pathway in cultured mammalian cells: Effect of substitution of galactose for glucose as the sole hexose in the medium in human diploid cell strains and in a rat hepatoma line, J. Cell. Physiol. 86: 91–103.
Stone, E. M., Rothblum, K. N., and Schwartz, R. J., 1985, Intron-dependent evolution of chicken glyceraldehyde phosphate dehydrogenase gene, Nature 313: 498–500.
Stone, K. R., Smith, R. E., and Joklik, W. K., 1974, Changes in membrane polypeptides that occur when chick embryo fibroblasts and NRK cells are transformed with avian sarcoma viruses, Virology 58: 86–100.
Sun, N. C., Chang, C. C., and Chu, E. H. Y., 1975, Mutant hamster cells exhibiting a pleiotropic effect on carbohydrate metabolism, Proc. Natl. Acad. Sci. USA 72: 469–473.
Tschopp, A., and Cogoli, A., 1983, Hypergravity promotes cell proliferation, Experientia 12: 1323–1329.
Usanga, E. A., and Luzzatto, L., 1985, Adaption of Plasmodium falciparum to glucose 6-phosphate dehydrogenase-deficient host red cells by production of parasite-encoded enzyme, Nature 313: 793–795.
Venetianer, A., and Bosze, Z., 1983, Expression of differentiated functions in dexamethasoneresistant hepatoma cells, Differentiation 25: 70–78.
Vozdev, V. A., 1976, Role of the pentose phosphate pathway in metabolism of D. melanogaster elucidated by mutations affecting glucose 6-phosphate and 6-phosphate gluconate dehydrogenase, FEBS Lett. 64: 85–88.
Wagner, K. R., Kauffman, F. C., and Max, S. R., 1978, The pentose phosphate pathway in regenerating skeletal muscle, Biochem. J. 170: 17–22.
Walker, D. G., 1966, The nature and function of hexokinases in animal tissues, in: Essays in Biochemistry, Vol. 2 ( P. N. Campbell and G. D. Greville, eds.), pp. 33–67, Academic Press, New York.
Wang, T., Marquardt, C., and Foker, J., 1976, Aerobic glycolyses during lymphocyte proliferation, Nature 261: 702–705.
Wang, T., Foker, J. E., and Tsai, M. Y., 1980, The shift of an increase in phosphofructokinase activity from protein synthesis-dependent to -independent mode during concanavalin A induced lymphocyte proliferation, Biochem. Biophys. Res. Commun. 95: 13–19.
Webber, M. J., Evans, P. K., Johnson, M. A., McNair, T. S., Nakamura, K. D., and Salter, D. W., 1984, Transport of potassium, amino acids, and glucose in cells transformed by Rous sarcoma virus, Fed. Proc. 43: 107–112.
Whitfield, C. D., Buchsbaum, B., Bostedor, R., and Chu, E. H. Y., 1978, Inverse relationship between galactokinase activity and 2-deoxygalactose resistance in Chinese hamster ovary cells, Somat. Cell Genet. 4: 699–713.
Williams, J. F., 1980, A critical examination of the evidence for the reactions of the pentose pathway in animal tissue, Trends Biochem. Sci. 5: 315–320.
Williams, J. F., Arora, K. K., and Longenecker, J. F., 1983, The F-pentose cycle doesn’t have the answers for liver tissue, Trends Biochem. Sci. 8: 275–277.
Wohlhueter, R. M., and Plagemann, P. G. W., 1981, Hexose transport and phosphorylation by Novikoff rat hepatoma cells as a function of extracellular pH, J. Biol. Chem. 256: 869–875.
Wolfrom, C., Loriette, C., Polini, G., Delhotal, B., Lemonnier, F.; and Gautier, M., 1983, Comparative effects of glucose and fructose on growth and morphological aspects of cultured skin fibroblasts, Exp. Cell Res. 149: 535–546.
Yoshida, A., and Miwa, S., 1974, Characterisation of a phosphoglycerate kinase variant associated with haemolytic anaemia, Am. J. Hum. Genet. 26: 378–384.
Ziegler, M. L., and Davidson, R. L., 1979, The effect of hexose on chloramphenicol sensitivity and resistance in Chinese hamster cells, J. Cell. Physiol. 98: 627–637.
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Morgan, M.J., Faik, P. (1986). The Utilization of Carbohydrates by Animal Cells. In: Morgan, M.J. (eds) Carbohydrate Metabolism in Cultured Cells. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7679-8_2
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