Skip to main content
SpringerLink
Log in
Book cover

Advances in Tracer Methodology pp 187–202Cite as

Application of Tracers to Quantitative Histochemical and Cytochemical Studies

  • Chapter

Abstract

The biochemist, concerned with metabolic characteristics of specific cell types or of specific subcellular organelles, is all too frequently limited by the sensitivity of the methods for the assay of a particular chemical or enzyme constituent. This limitation in sensitivity is a function of many variables and would include (1) the inherent sensitivity of the physical technique used for measurements (pH, absorbance, fluorescence, radioactivity, and the like), (2) the concentration or activity of the component being studied, and (3) the size of tissue sample available for study (gram vs. microgram). The latter points may apply particularly to the problems encountered in an attempt to study the enzymatic and chemical constituents of cell types present in the nervous system [1–4].

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Lowry, O.H., in Waelsch, H. (editor), Biochemistry of the Developing Nervous System, Academic Press, New York, 1954.

    Google Scholar 

  2. Lowry, O.H., in Cohen, M., and Snider, R. (editors), Morphological and Biochemical Correlates of Neural Activity, Harper & Row, New York, 1964.

    Google Scholar 

  3. Robins, E., Exp. Cell Res. Suppl. 4: 241 (1957).

    Google Scholar 

  4. Robins, E., Smith, D.E., Eydt, K.M., and McCaman, R.E., J. Neurochem. 1: 68 (1956).

    Article  CAS  Google Scholar 

  5. Lowry, O.H., Roberts, N.R., Wu, M-L., Hixon, W.S., and Crawford, E.J., J. Biol. Chem. 207: 19 (1954).

    CAS  Google Scholar 

  6. Robins, E., Smith, D.E. and McCaman, R.E., J. Biol. Chem. 204: 927 (1953).

    CAS  Google Scholar 

  7. Glick, D., in Glick, D. (editor), Quantitative Chemical Techniques of Histo-and Cytochemistry, John Wiley, New York, 1961.

    Google Scholar 

  8. Lowry, O.H., Roberts, N.R., and Kapphahn, J.I., J.Biol. Chem. 224: 1047 (1957).

    CAS  Google Scholar 

  9. Lowry, O.H., Passonneau, J.V., Hasselberger, F.X., and Schulz, D.W., J. Biol. Chem. 239: 18 (1964).

    CAS  Google Scholar 

  10. Lowry, O.H., Passonneau, J.V., Schulz, D.W., and Rock, M.K., J. Biol. Chem. 236: 2746 (1961).

    CAS  Google Scholar 

  11. McCaman, R.E., McCaman, M.W., Hunt, J.M., and Smith, M.S., J. Neurochem. 12: 15 (1965).

    Article  CAS  Google Scholar 

  12. Axelrod, J., Albers, W., and Clemente, C.D., J. Neurochem. 5: 68 (1959).

    Article  CAS  Google Scholar 

  13. McCaman, R.E., Life Sci. 4: 2353 (1965).

    Article  CAS  Google Scholar 

  14. Axelrod, J., and Tomchick, R.J., J. Biol. Chem. 233: 702 (1958).

    CAS  Google Scholar 

  15. McCaman, R.E., and Cook, K., J. Biol. Chem. 241: 3390 (1966).

    CAS  Google Scholar 

  16. McCaman, R.E., and Cook, K., in preparation.

    Google Scholar 

  17. McCaman, R.E., in preparation.

    Google Scholar 

  18. McCaman, M.W., and McCaman, R.E., in preparation.

    Google Scholar 

  19. Albers, R.W., and Brady, R.O., J. Biol. Chem. 234: 926 (1959).

    CAS  Google Scholar 

  20. Pastan, I., Wills, V., Herring, B., and Field, J.B., J. Biol. Chem. 238: 3362 (1963).

    CAS  Google Scholar 

  21. Schuberth, J., Acta Chem. Scand. 17, Suppl. 1: S233 (1963).

    Google Scholar 

  22. McCaman, R.E., and Hunt, J.M., J. Neurochem., 12: 253 (1965).

    Article  CAS  Google Scholar 

  23. McCaman, R.E., McCaman, M.W., and Stafford, M.L., J. Biol. Chem. 241: 930 (1966).

    CAS  Google Scholar 

  24. Fuller, R.W., and Hunt, J.M., Anal. Biochem. 16: 349 (1966).

    Article  CAS  Google Scholar 

  25. Baldessarini, R.J., and Kopin, I.J., Anal. Biochem. 6: 289 (1963).

    Article  CAS  Google Scholar 

  26. Baldessarini, R.J., and Kopin, I.J., J. Neurochem. 13: 769 (1966).

    Article  CAS  Google Scholar 

  27. Snyder, S.H., Baldessarini, R.J., and Axelrod, J., J. Pharmacol. Exp. Therap. 153: 544 (1966).

    CAS  Google Scholar 

  28. Lowry, O.H., Roberts, N.R., and M-L.W. Chang, J. Biol. Chem. 222: 97 (1956).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA

    Richard E. McCaman

Authors
  1. Richard E. McCaman
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. New England Nuclear Corporation, Boston, Massachusetts, USA

    Seymour Rothchild

Rights and permissions

Reprints and permissions

Copyright information

© 1968 New England Nuclear Corporation

About this chapter

Cite this chapter

McCaman, R.E. (1968). Application of Tracers to Quantitative Histochemical and Cytochemical Studies. In: Rothchild, S. (eds) Advances in Tracer Methodology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7532-6_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-7532-6_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7534-0

  • Online ISBN: 978-1-4684-7532-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation