Changes in Kininogen Content of Serum and Some Organs during Injury and Inflammation in Rats

Abstract

We have explored some components of the kallikrein-kinin-system after injury and inflammation in rats. Prekallikrein, kininogen and kininase-activity in serum were estimated by following methods: prekallikrein was activated by trypsin (1 mg/ml) and tested against pancreas kallikrein on the blood pressure of anaesthetized dogs. To estimate serum kininogen heatacid-denatured serum was incubated with trypsin (2mg/ ml, 25 min at 37°C); then the samples were chromatographed on Amberlite IRP-64 and the eluats were tested against synthetic bradykinin on the rat uterus. Sometimes the amounts of kinin-activity of the eluats were controlled on the blood-pressure of anaesthetized dogs. Only such smooth muscle activity was regarded as a kinin activity which was inactivated by incubation with chymotrypsin, carboxypeptidase B and native serum. To estimate kininase-activity serum was diluted with tris-buffer and incubated with bradykinin. Then that moment was determined, at which 50% of added bradykinin were inactivated.