Summary
It was found that by using nonlinear multicomponent analysis with the spectra of oxygenated and deoxygenated haemoglobin as basic spectra the mean O2 saturation of 2 to 3 red cells lying in a rouleau could be determined with an accuracy of 0.5–1.0%. At the same PO2 and PCO2 distinct differences in O2 saturation were found in different red cells; for 50% of the saturation values the differences were in the range ± 1.5%, for 30% of the values, the differences were in the range of ± 3% and 15% lay in the range of ± 4.5%. Only 5% of saturation differences were larger than 4.6%. These differences are so large that they have to be considered in calculations of the PO2 from O2 saturation measurements.
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© 1987 Plenum Press, New York
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Lubbers, D.W., Grisar, H., Gayeski, T.E.J. (1987). The Microphotometric Determination of the Variability of Oxygen Saturation of Erythrocytes Lying within Rouleaux. In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_5
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DOI: https://doi.org/10.1007/978-1-4684-7433-6_5
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