Abstract
It is well-known that the cerebral cortex is heterogeneously supplied with oxygen. According to microelectrode measurements, PO2 at some microregions of the brain cortex is close to zero mmHg (Silver, 1966; Metzger and Heuber, 1977). Supporting these data, Rosenthal et al. (1976) showed that the oxido-reduction state of cytochrome a,a3 in the brain cortex is shifted toward a more oxidized state if cerebral oxygen supply is increased. On the basis of this finding, Rosenthal et al. (1976), in accordance with Davies and Bronk (1957), suggested that under physiological conditions the brain cortex is on the border of slight hypoxia. This assumption seemed to be supported by the data of Kontos et al. (1978) and Morii, Winn and Berne, (1983). They showed that the autoregulatory dilatation of pial arteries can be counteracted by superfusion of the brain cortex with oxygen-saturated mock cerebrospinal fluid (CSF) or fluorocarbon (Kontos et al., 1978), and the resting cerebral blood flow (CBF) can be decreased by systemic theophylline treatment (Morii et al., 1983). However, other investigators (Leniger-Follert, 1985; Rubin and Bohlen, 1985) found no change in cortical PO2 during autoregulation, and resting CBF was not altered by systemic theophylline treatment in Emerson and Raymond’s (1981) experiments. This controversy in the literature led us to investigate the effects of an excess of oxygen on the steady NAD/NADH redox state and autoregulation of the brain cortex. In addition, we studied the effect of topical adenosine deaminase and theophylline treatment of the brain cortex in order to see whether adenosine (ADO) contributes to the maintenance of resting CBF.
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References
Davies, P.W. and Bronk, D.W. (1957). Oxygen tension in mammalian brain. Fed. Proc. 16, 689
Dora, E. (1984). A simple cranial window technique for optical monitoring of cerebrocortical microcirculation and NAD/NADH redox state. Effect of mitochondrial electron transport inhibitors and anoxic anoxia. J. Neurochem. 42, 101
Dora, E. (1985). Further studies on the reflectometric monitoring of cerebrocortical microcirculation. Importance of lactate anions in coupling between cerebral blood flow and metabolism. Acta Physiol. Hung. 66, 199
Dora, E. and Kovach, A.G.B. (1983). Effect of topically administered epinephrine, norepinephrine, and acetylcholine on cerebrocortical circulation and the NAD/NADH redox state. J. Cereb. Blood Flow Metabol. 3, 161
Edvinsson, L. and Owman, C. (1977). Pharmacological characterization of postsynaptic vasomotor receptors in brain vessels. In: Neurogenic Control of Brain Circulation. Eds Owman, C. and Edvinsson, L., Pergamon Press, New York, p. 167
Eke, A., Hutiray, Gy. and Kovach, A.G.B. (1979). Induced hemodilution detected by reflectometry for measuring microregional blood flow and blood volume in cat brain cortex. Am. J. Physiol. 236, H759
Emerson, T.E. and Raymond, R.M. (1981). Involvement of adenosine in cerebral hypoxic hyperemia in dog. Am.J.Physiol. 241, H134
Furchgott, R.F. (1983). Role of endothelium in responses of vascular smooth muscle. Circ. Res. 53, 557
Furchgott, R.F. and Zawadski, J.V. (1980). The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature, 288, 273
Gibson, G.E., Duffy, T.E. and Plum, F. (1980). Acetylcholine synthesis and cerebral blood flow during hypoxaemia. In: Cerebral Circulation and Neurotransmitters. Eds Bes, A. and Geraud, G., Excerpta Medica, Amsterdam, p. 199
Hogestatt, E.D., Andersson, K.-E. and Edvinsson, L. (1983). Mechanical properties of rat cerebral arteries as studied by a sensitive device for recording of mechanical activity in isolated small blood vessels. Acta Physiol. Scand. 117, 49
Kontos, H.A., Wei, E.P., Raper, A.J., Rosenblum, W.I., Navari, R.M. and Patterson, J.L. (1978). Role of tissue hypoxia in local regulation of cerebral microcirculation. Am. J.Physiol. 234, H582
Lee, T.J.F. (1981). Is acetylcholine the dilator transmitter in cerebral blood vessels? A critical examination. J. Cereb. Blood Flow Metabol. 1 (Suppl), S305
Leniger-Follert, E. (1985). Oxygen supply and microcirculation of the brain cortex. In: Oxygen Transport to Tissue-VII. Eds Kreuzer, F., Cain, S.M., Turek, Z. and Goldstick, T.K., Plenum Press, New York and London, ( Adv. Exp. Med. Biol. 191, 3 ).
Metzger, H. and Heuber, S. (1977). Local oxygen tension and spike activity of the cerebral grey matter of the rat and its response to short intervals of 02 deficiency or CO2 excess. Pflugers Arch. 370, 201
Morii, S., Winn, H.R. and Berne, R.M. (1983). Effect of theophylline, an adenosine receptor blocker, on cerebral blood flow (CBF) during rest and transient hypoxia. J. Cereb. Blood Flow Metabol. 3 (Suppl), S480
Rosenthal, M., LaManna, J.C., Jobsis, F.F., Levasseur, J.E., Kontos, H.A. and Patterson, J.L. (1976). Effects of respiratory gases on cytochrome a in intact cerebral cortex: Is there a critical P02? Brain Res. 108, 143
Rubin, M.J. and Bohlen, H.G. (1985). Cerebral vascular autoregulation of blood flow and tissue P02 in diabetic rats. Am. J. Physiol. 249, H540
Silver, I.A. (1966). The measurement of oxygen tension in tissues. In: Oxygen Measurements in Blood and Tissues and their Significance. Eds Payne, J.P. and Hill, D.W., Churchill, London, p. 135
Winn, R.H., Rubio, G.R. and Berne, R.M. (1981). The role of adenosine in the regulation of cerebral blood flow. J. Cereb. Blood Flow Metabol. 1, 239
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© 1987 Plenum Press, New York
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Dora, E., Kovach, A.G.B. (1987). Role of Hypoxia and Acetylcholine in the Regulation of Cerebral Blood Flow. In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_25
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DOI: https://doi.org/10.1007/978-1-4684-7433-6_25
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