Skip to main content
SpringerLink
Log in

Cytochrome P-450 and Vitamin E Free Radical Reductase: Formation of and Protection Against Free Radicals

  • Chapter
Free Radicals, Lipoproteins, and Membrane Lipids

Part of the book series: NATO ASI Series ((NSSA,volume 189))

Summary

In studies on lipid peroxidation, liver microsomes are frequently employed. Already in 1963, Hochstein and Ernstere1 reported that in their experiments lipid peroxidationin rat liver microsomes required molecular oxygen and NADPH and that lipid peroxidation intensified by the availability of ADP and Fe2+. This process is referred to as enzymic lipid peroxidation since it appears to depend on the presence of the haemoprotein cytochrome P-450 and the flavoprotein NADPH cytochrome P-450 reductase, both residing in the microsomal membrane2. The precise contribution of both proteins to NADPH dependent microsomal lipid peroxidation is still a matter of debate2. We described that cytochrome P-450 might be involved in both lipid hydroperoxide (LOOH) independent and LOOH dependent lipid peroxidation2.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. P. Hochstein and L. Ernster, ADP-activated lipid peroxidation coupled to the TPNH oxidase system of microsomes. Biochem, Biophys. Res, Commun. 12: 388 (1963).

    Article  CAS  Google Scholar 

  2. A. Bast and G.R.M.M. Haenen, Cytochrome P-450 and glutathione: What is the significance of their interrelationship in lipid peroxidation? Trends Biochem. Sci. 9: 510 (1984).

    Article  CAS  Google Scholar 

  3. A. Bast, Is formation of reactive oxygen by cytochrome P-450 perilous and predictable? Trends Pharmacol. Sci. 7: 266 (1986).

    Article  CAS  Google Scholar 

  4. G. R.M.M. Haenen and A. Bast, Protection against lipid peroxidation by a microsomal glutathione-dependent labile factor. FEBS Lett. 159: 24 (1983).

    Article  CAS  PubMed  Google Scholar 

  5. D. W. Nebert, M. Adesnik, M.J. Coon, R.W. Estabrook, F.J. Gonzalez, F.P. Guengerich, I.C. Gunsalus, E.F. Johnson, B. Kemper, W. Levin, I.R. Phillips, R. Sato and M.R. Waterman, The P-450 gene superfamily: recommended nomenclature. DNA 6: 1 (1987).

    PubMed  CAS  Google Scholar 

  6. Y. Terelius and M. Ingelman-Sundberg, Cytochrome P-450-dependent oxidase activity and hydroxyl radical production in micellar and membranous types of reconstituted systems. Biochem. Pharmacol, 37: 1383 (1988).

    Article  CAS  PubMed  Google Scholar 

  7. G. Powis and I. Jansson, Stoichiometry of the mixed function oxidase. Pharmac. Ther. 7: 297 (1979).

    Article  CAS  Google Scholar 

  8. L. D. Gorsky, D.R. Koop and M.J. Coon, On the stoichiometry of the oxidase and monooxygenase reactions catalyzed by liver microsomal cytochrome P-450. Products of oxygen production. J. Biol. Chem. 259: 6812 (1984).

    PubMed  CAS  Google Scholar 

  9. A. Bast, E.M. Savenije-Chapel and B.H. Kroes, Inhibition of mono-oxygenase and oxidase activity of reat-hepatic cytochrome P-450 by H2-receptor blockers. Xenobiotica 14: 399 (1984).

    Article  CAS  PubMed  Google Scholar 

  10. A. Bast, J.W. Brenninkmeijer, E.M. Savenije-Chapel and J. Noordhoek, Cytochrome P-450 oxidase activity and its role in NADPH dependent lipil peroxidation. FEBS Lett. 151: 185 (1983).

    Article  CAS  PubMed  Google Scholar 

  11. A. Bast and M.H.M. Steeghs, Hydroxyl radicals are not involved in NADPH dependent microsomal lipid peroxidation. Experientia 42: 555 (1986).

    Article  CAS  PubMed  Google Scholar 

  12. L. A. Morehouse, M. Tien, J.R. Bucher and S.D. Aust, Effect of hydrogen peroxide on the initiation of microsomal lipid peroxidation. Biochem, Pharmacol. 32: 123 (1983).

    Article  CAS  Google Scholar 

  13. G. Minotti and S.D. Aust, The role of iron in the initiation of lipid peroxidation. Chem. Physics of Lipids 44: 191 (1987).

    Article  CAS  Google Scholar 

  14. H. Wefers and H. Sies, The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E. Eur. J. Biochem. 174: 353 (1988).

    Article  CAS  Google Scholar 

  15. A. Bast, G.R.M.M. Haenen and E.M. Savenije-Chapel, Inhibition of rat hepatic microsomal lipid peroxidation by mesna via glutathione. Arzneim.-Forsch./Drug Res. 37: 1043 (1987).

    CAS  Google Scholar 

  16. L. G. Forni and R.L. Willson, Vitamin C and consecutive hydrogen atom and electron transfer reactions in free radical protection: A novel catalytic role for glutathione, in: “Protective agents in cancer”, D.C. McBrien and T.F. Slater eds., Academic Press, New York (1983).

    Google Scholar 

  17. R. Morgenstern, G. Lundqvist, V. Hancock and J.W. DePierre, Studies on the activity and activation of rat liver microsomal glutathione transferase, in particular with a substrate analogue series. J, Biol. Chem, 263: 6671 (1988).

    CAS  Google Scholar 

  18. E. Mousialou and R. Morgenstern, Studies on the glutathione dependent inhibition of lipid peroxidation. Abstract 3.15 in Eur. Workshop on Drug Metab., Univ. of Konstanz, F.R.G. (1988).

    Google Scholar 

  19. G. R.M.M. Haenen, J.N.L. Tai Tin Tsoi, N.P.E. Vermeulen, H. Timmerman and A. Bast, 4-Hydroxy-2,3-trans-nonenal stimulates microsomal lipid peroxidation by reducing the gluthathione-dependent protection. Arch, Biochem. Biophys, 259: 449 (1987).

    Article  CAS  Google Scholar 

  20. R. Morgenstern, H. Wallin and J.W. DePierre, Mechanisms of activation of the microsomal glutathione transferase, in “Glutathione S-transferases and carcinogenesis”, T.J. Mantle, C.B. Pickett and J.D. Hayes eds., Taylor and Francis, London, New York and Philadelphia (1987).

    Google Scholar 

  21. R. F. Burk, Glutathione-dependent protection by rat liver microsomal protein against lipid peroxidation. Biochim. Biophys. Acta 757: 21 (1983).

    CAS  Google Scholar 

  22. M. Yonaha and Y. Tampo, Studies on protection by glutahione against lipid peroxidation in rat liver microsomes. Effect of bromosulfophtalein. Chem, Pharm. Bud. 34: 4195 (1986).

    CAS  Google Scholar 

  23. F. Ursini, M. Maiorino, M. Valente, L. Ferri and C. Gregolin. Purification from pig liver of a protein which protects liposomes and biomembranes from peroxidative degradation and exhibits glutathione peroxidase activïiy on phosphatidylcholine hydroperoxides. Biochim. Biophys. Acta 710: 197 (1982).

    Article  CAS  PubMed  Google Scholar 

  24. F. Ursini and A. Bindoli, The role of selenium peroxidases in the protection against oxidative damage of membranes. Chem. Physics of Lipids. 44: 255 (1987).

    Article  CAS  Google Scholar 

  25. C. C. Reddy, R.W. Scholz, C.E. Thomas and E.J. Massaro, Vitamin E dependent reduced glutathione inhibition of rat liver microsomal lipid peroxidation. Life Sci. 31: 571 (1982).

    CAS  Google Scholar 

  26. M. E. Murphy and J.P. Kehrer, Simultaneous measurement of tocopherols and tocopheryl quinones in tissue fractions using high-performance liquid chrom atography with redoxcycling electrochemical detection. J. Chromatogr. (Biomed. Anplicat.) 421: 71 (1987).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Phannacochemistry, Faculty of Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

    Aalt Bast & Guido R. M. M. Haenen

Authors
  1. Aalt Bast
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guido R. M. M. Haenen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. INSERM Unit 58, Montpellier, France

    A. Crastes de Paulet

  2. INSERM Unit 101, Toulouse, France

    L. Douste-Blazy

  3. University of Milan, Milan, Italy

    R. Paoletti

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Plenum Press, New York

About this chapter

Cite this chapter

Bast, A., Haenen, G.R.M.M. (1990). Cytochrome P-450 and Vitamin E Free Radical Reductase: Formation of and Protection Against Free Radicals. In: de Paulet, A.C., Douste-Blazy, L., Paoletti, R. (eds) Free Radicals, Lipoproteins, and Membrane Lipids. NATO ASI Series, vol 189. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7427-5_33

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-7427-5_33

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-7429-9

  • Online ISBN: 978-1-4684-7427-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation