Abstract
The combination of a harmless chemical photoactivated with safe visible electromagnetic radiation in order to eradicate tumors with a photo-sensitization reaction, offers much hope for the treatment of a large variety of neoplastic diseases. The origin for the selective biodistribution of photosensitizer toward tumor tissue is not yet clearly understood. The pH of many rapidly growing tumors is often found to be significantly lower than that of normal tissue in the same individual. Recent volumetric titration of hematoporphyrin IX (HP) have yielded the distribution diagrams of five HP ionic species, having net charges of +2, +1, 0, -1 and -2. However, only three ionic species may be associated with the steady state absorbance and fluorescence spectra. Further, fluorescence decay measurements carried out in aqueous solutions at physiological pH generally show two lifetimes; a 15 nsec component normally ascribed to a monomeric species and a 3.8 nsec component usually assigned to a dimeric species . Since both monomeric and dimeric species, as well as different ionic species, may play a role in the selective biodistribution of drugs toward tumor tissue, it was deemed important to evaluate the fluorescence lifetimes of HP as a function of both pH and concentration. These measurements were carried out in aqueous solutions as well as in the presence of a surfactant, sodium dodecyl sulfate (SDS).
Chapter PDF
Similar content being viewed by others
Keywords
- Ionic Species
- Fluorescence Lifetime
- Volumetric Titration
- Sodium Dodecyl Sulfate Concentration
- Monomeric Species
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
W. J. Waddell and R. G. Bates, “Intercellular pH”, Physiol. Rev., 49:285 (1969)
T. C. Ng, W. T. Evanochko, R. N. Hiramoto, V. K. Ghanta, M. B. Lilly, A. J. Lawson, T. H. Corbet, J. R. Durant and J. D. Glickson, “31P NMR spectroscopy of in vivo tumors”, J. Magn. Reson., 49:271 (1982)
J. L. Wike-Hooley, J. Haveman and H. S. Reinhold, “The relevance of tumor pH to the treatment of malignant diseases”, Radiother. Oncol. 2:366 (1984)
A. J. Thistlethewaite, D. B. Leeper, D. J. Moylar III and R. E. Nerlinger, “pH distribution in human tumors”, Int. J. Radiat. Oncol. Biol. Phys., 11:1647 (1985)
A. J. Barrett, J. C. Kennedy, R. A. Jones, P. Nadeau and R. H. Pottier, “The effect of tissue and cellular pH on the selective biodistribution of porphyrin-type photochemotherapeutic agents: A volumetric titration study”, J. Photochem. Photobiol. (B:Biology) 6:1 (1990)
R. H. Pottier, J. C. Kennedy, Y. F. A. Chow and F. Cheung, “The pK values of hematoporphyrin IX as determined by absorbance and fluorescence spectroscopy”, Can. J. Spectrosc. 33(3):57 (1988)
A. Andreoni, R. Cubeddu, S. De Silvestri, G. Jori, P. Laporta and E. Reddi, “Time-resolved fluorescence studies of hematoporphyrin in different solvent systems”, Z. Naturforsch. 38c:83 (1983)
C. Vever-Bizet, O. Delgado and D. Brault, “The purification of hematoporphyrin IX and its acetylated derivatives”, J. Chromatog., 283:157 (1984)
M. Dellinger and D. Brault, “Normal-phase high performance liquid chromatography of free acid dicarboxylic porphyrin and hematopor-phyirin derivative on silica, J. Chromatog. 422:73 (1987)
M. Zuker, A. G. Szabo, L. Bramall, D. T. Krajcarsk and B. Sellinger, “Delta function convolution method (9DFCM) for fluorescence decay experiments”, Rev. Sci. Instrum. 56(1):14 (1985)
J. S. Sun, M. Rouge, M. Delarue, T. Montenay-Garestier and C. Helene, “Solvent Relaxation around Excited 2-Methoxy-6-chloro-9-amino-acridine in aqueous solvents”, J. Phys. Chem. 94:968 (1990)
M. Yamashita, M. Nomura, S. Kobayashi, T. Sato and K. Aizawa, “Pico-second time-resolved fluorescence spectroscopy of hematoporphyrin derivative”, IEEE J. Quant. Electr. QE-20 (12):1363 (1984)
J. P. Thomas and A. W. Girotti, “Glucose administration augments in vivo uptake and phototoxicity of the tumor-localizing fraction of hematoporphyrin derivative, Photochem. Photobiol. 49(3):241 (1989)
R. Pottier, A. Lachaine, M. Pierre and J. C. Kennedy, “A new electronic absorbance band in concentrated aqueous solutions of hematoporphyrin IX detected by photoacoustic spectroscopy”, Photochem. Photobiol. 47(5):669 (1988)
D. Kessel, “Hematoporphyrin and HPD: photophysics, photochemistry and phototherapy”, Photochem. Photobiol. 39:851 (1984)
P. Seeman and H. Kalant, “Drug solubility, K absorption, and movement across body membranes”, in “Principles of Medical Pharmacology”, H. Kalant, W. H. E. Roschian and E. M. Sellers (eds.), University of Toronto Press, Toronto, 4th edn., p. 11 (1985)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Nadeau, P., Pottier, R.H., Szabo, A., Brault, D., Vever-Bizet, C. (1991). Laser Excited Fluorescence Lifetimes of Hematoporphyrin IX as a Function of Solution pH: Implication in Photodynamic Therapy. In: Chester, A.N., Martellucci, S., Scheggi, A.M. (eds) Laser Systems for Photobiology and Photomedicine. NATO ASI Series, vol 252. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7287-5_23
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7287-5_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7289-9
Online ISBN: 978-1-4684-7287-5
eBook Packages: Springer Book Archive