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Membrane Topology of Cytochromes P-450: Elements and Measurement by Spectroscopic Techniques

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Molecular Aspects of Monooxygenases and Bioactivation of Toxic Compounds

Part of the book series: NATO ASI Series Advanced Science Institutes Series ((NSSA,volume 202))

Abstract

The main concern of these lectures is: Do liver microsomal cytochromes P-450 have an oligomeric structure and is cooperativity involved in their function? Elements of topology of membrane proteins and the spectroscopic techniques used to investigate this toplogy are described. Oligomeric structure is an important element of membrane topology and information on it can be obtained from measurements of rotational diffusion of the proteins. Emphasis is therefore laid on photoselection techniques (time-dependent polarized delayed fluorescence and phosphorescence and time-dependent absorption dichroism).

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Authors and Affiliations

  1. Max-Planck-Institut für biophysikalische Chemie, Abteilung Spektroskopie, D-3400, Göttingen, Germany

    A. Stier, V. Krüger, T. Eisbein & S. A. E. Finch

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  1. A. Stier
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  2. V. Krüger
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  3. T. Eisbein
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  4. S. A. E. Finch
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Editor information

Editors and Affiliations

  1. Middle East Technical University, Ankara, Turkey

    Emel Arinç

  2. University of Connecticut Health Center, Farmington, Connecticut, USA

    John B. Schenkman

  3. North Carolina State University, Raleigh, North Carolina, USA

    Ernest Hodgson

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© 1991 Plenum Press, New York

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Stier, A., Krüger, V., Eisbein, T., Finch, S.A.E. (1991). Membrane Topology of Cytochromes P-450: Elements and Measurement by Spectroscopic Techniques. In: Arinç, E., Schenkman, J.B., Hodgson, E. (eds) Molecular Aspects of Monooxygenases and Bioactivation of Toxic Compounds. NATO ASI Series Advanced Science Institutes Series, vol 202. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7284-4_6

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  • DOI: https://doi.org/10.1007/978-1-4684-7284-4_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7286-8

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