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Gastrointestinal Hormones and Pathology of the Digestive System pp 317–318Cite as

Radioimmunoassay of Vasoactive Intestinal Polypeptide (VIP) in Plasma

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 106))

Abstract

A sensitive and specific radioimmunoassay for VIP has been developed which can detect 3.3 pmol liter-1 of the peptide in plasma1. Antisera to highly purified porcine VIP covalently coupled to bovine serum albumin by the use of carbodiimide were raised in eight rabbits. The immunization dose was 15 nmol (50 μg) VIP per rabbit and injections were given subcutaneously at 8 week1 intervals. The final dilution, the avidity, and the specificity of each antiserum were determined.

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References

  1. Fahrenkrug J, Schaffalitzky de Muckadell OB: Radioimmunoassay of vasoactive intestinal polypeptide (VIP) in plasma. J. Lab. Clin. Med. 89, 1379–1388, 1977

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  2. Schaffaltizky de Muckadell OB, Fahrenkrug J, Holst JJ, Lauritsen KB: Release of vasoactive intestinal polypeptide (VIP) by intraduodenal stimuli. Scand. J. Gastroent., in press

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  3. Schaffalitzky de Muckadell OB, Fahrenkrug J, Holst JJ: Release of vasoactive intestinal polypeptide (VIP) by electric stimulation of the vagal nerves. Gastroenterology 72, 373–375, 1977

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Author information

Authors and Affiliations

  1. Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark

    J. Fahrenkrug & O. B. Schaffalitzky De Muckadell

Authors
  1. J. Fahrenkrug
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  2. O. B. Schaffalitzky De Muckadell
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Editor information

Editors and Affiliations

  1. Veterans Administration, Wadsworth Hospital Center, Los Angeles, California, USA

    Morton Grossman

  2. Institute of 3rd Surgical Pathology, University of Rome, Italy

    V. Speranza , N. Basso  & E. Lezoche ,  & 

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© 1978 Plenum Press, New York

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Fahrenkrug, J., De Muckadell, O.B.S. (1978). Radioimmunoassay of Vasoactive Intestinal Polypeptide (VIP) in Plasma. In: Grossman, M., Speranza, V., Basso, N., Lezoche, E. (eds) Gastrointestinal Hormones and Pathology of the Digestive System. Advances in Experimental Medicine and Biology, vol 106. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7248-6_39

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  • DOI: https://doi.org/10.1007/978-1-4684-7248-6_39

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7250-9

  • Online ISBN: 978-1-4684-7248-6

  • eBook Packages: Springer Book Archive

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