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In Vitro Manipulation of Barley and Other Cereals

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Gene Manipulation in Plant Improvement II

Abstract

Already, it can be seen and more so in the future, it is expected, that plant cell biology and molecular biology will have a major impact on agriculture by supplementing the present activities of plant breeders in expanding and diversifying the gene pool of crop species and in speeding up the breeding process. Different strategies are used which apply in vitro methods to generate diversity within existing populations, to identify rare, but desired individual plants, and to broaden the genetic pool of breeding material. Of major interest are the production of homozygous lines by anther- and micropsore-culture, the in vitro selection of cultured cells to create stress or disease resistant plants, protoplast fusion and somatic hybridization to overcome the natural barriers of incompatibility or to establish new combinations of organellar and nuclear genomes in somatic hybrid or cybrid plants, and finally the transfer of isolated genes to achieve a directed, highly defined genetic modification of a specific crop plant. Progress with cereals and grasses in the past has been rather slow when compared to species such as Nicotiana tabacum, Solanum tuberosum or Brassica napus. The field has been reviewed recently in numerous articles (Göbel and Lörz, 1988; Lörz et al., 1988; Ozias-Akins and Vasil, 1988), thus mostly recent experiments from our laboratory and new developments will be discussed.

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© 1990 Plenum Press, New York

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Lörz, H. et al. (1990). In Vitro Manipulation of Barley and Other Cereals. In: Gustafson, J.P. (eds) Gene Manipulation in Plant Improvement II. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7047-5_9

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  • DOI: https://doi.org/10.1007/978-1-4684-7047-5_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7049-9

  • Online ISBN: 978-1-4684-7047-5

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