Abstract
Immunofluorescent techniques have been used by several investigators to study the replication of SV40 virus (1–3); in all cases, antiviral immune serum was used, which would detect antigens on the surface of the viral particle. In African green monkey kidney cell cultures (AGMK) infected with SV40 virus, viral antigen appeared in the nucleus at 18 hours and reached a peak at 38 hours, after which nuclear antigen decreased and a cytoplasmic stage occurred (1). Nuclear fluorescence was also observed 10 days after infection of cells of grivet monkey kidney, rhesus monkey kidney, human embryo kidney, and Syrian hamster kidney (2). The same techniques were applied to cells transformed by SV40 virus in a search for evidence of viral antigen. Faint nuclear fluorescence, interpreted as non-specific, was observed in transformed Syrian hamster kidney cells (2). In cultures of transformed human embryo kidney cells, which were producing virus, less than 0.1 per cent of cells showed nuclear fluorescence (3) and it seems probable that these were the cells responsible for virus replication.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Bibliography
Mayor, H. D., Stinebaugh, S. E., Jamison, R. M., Jordan, L. E., and Melnick, J. L., Immunofluorescent, cytochemical, and microcytological studies on the growth of the simian vacuolating virus (SV-40) in tissue culture, Exp. Mol. Path., 1962, 1, 397.
Levinthal, J. D., and Shein, H. M., Morphology and distribution of SV40-infected cells in human, simian and hamster renal cell cultures as detected by immunofluorescence, Proc. Soc. Exp. Biol. and Med., 1963, 112, 405.
Shein, H. M., Enders, J. R., and Levinthal, J. D., Transformation induced by simian virus 40 in human renal cell cultures. II. Cell-virus relationships, Proc. Nat. Acad. Sc., 1962, 48, 1350.
Black, P. H., Rowe, W. P., Turner, H. C., and Huebner, R. J., A specific complement-fixing antigen present in SV40 tumor and transformed cells, Proc. Nat. Acad. Sc., 1963, 50, 1148.
Black, P. H., and Rowe, W. P., An analysis of SV40-induced transformation of hamster kidney tissue in vitro. I. General characteristics, Proc. Nat. Acad. Sc., 1963, 50, 606.
Black, P. H., and Rowe, W. P., SV-40 induced proliferation of tissue culture cells of rabbit, mouse, and porcine origin, Proc. Soc. Exp. Biol. and Med., 1963, 114, 721.
Ashkenazi, A., and Melnick, J. L., Tumorigenicity of simian papovavirus SV40 and of virus-transformed cells, J. Nat. Cancer Inst., 1963, 30, 1227.
MacPherson, I., and Stoker, M., Polyoma transformation of hamster cell clonesan investigation of genetic factors affecting cell competence, Virology, 1962, 16, 147.
Hopps, H. E., Bernheim, B., Nisalak, A., and Smadel, J. E., Biological characteristics of a serially cultivated kidney cell culture derived from the African green monkey Cercopithecus aethiops, Fed. Proc., 1962, 21, 454.
Huebner, R. J., Rowe, W. P., Turner, H. C., and Lane, W. T., Specific adenovirus complement-fixing antigens in virus-free hamster and rat tumors, Proc. Nat. Acad. Sc., 1963, 50, 379.
Black, P. H., Rowe, W. P., and Cooper, H. L., An analysis of SV40-induced transformation of hamster kidney tissue in vitro. II. Studies of three clones derived from a continuous line of transformed cells, Proc. Nat. Acad. Sc., 1963, 50, 847.
Sabin, A. B., and Koch, M. A., Evidence of continuous transmission of noninfectious SV40 viral genome in most or all SV40 hamster tumor cells, Proc. Nat. Acad. Sc., 1963, 49, 304.
Melnick, J. L., Stinebaugh, S. E., and Rapp, F., Incomplete simian papovavirus SV40. Formation of non-infectious viral antigens in the presence of fluorouracil, J. Exp. Med., 1964, 119, 313.
Habel, K., and Eddy, B. E., Specificity of resistance to tumor challenge of polyoma and SV40 virus-immune hamsters, Proc. Soc. Exp. Biol. and Med., 1963, 113, 1.
Koch, M., and Sabin, A. B., Specificity of virus-induced resistance to transplantation of polyoma and SV40 tumors in adult hamsters, Proc. Soc. Exp. Biol. and Med., 1963, 113, 4.
Defendi, V., Effect of SV40 virus immunization on growth of transplantable SV40 and polyoma virus tumors in hamsters, Proc. Soc. Exp. Biol. and Med., 1963, 113, 12.
Editor information
Rights and permissions
Copyright information
© 1976 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pope, J.H., Rowe, W.P. (1976). Detection of Specific Antigen in SV40-Transformed Cells by Immunofluorescence. In: Schiminovich, S. (eds) The Biology of DNA Tumor Viruses. Milestones in Current Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6970-7_9
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6970-7_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6972-1
Online ISBN: 978-1-4684-6970-7
eBook Packages: Springer Book Archive