Abstract
It has been shown by a modified DNA-RNA hybridization technique that the nuclei of cells transformed by either polyoma virus or SV40 contain viral DNA.1 This method employs RNA synthesized in vitro with form I viral DNA2–4 and E. coli RNA polymerase. The number of viral DNA equivalents per cell varies from 5 to 60, depending on the cell line. In this communication, we will report on the physical state of the viral DNA in SV3T3 cells, an SV40-transformed cell line that contains 20 SV40 DNA equivalents per cell.
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Sambrook, J., Westphal, H., Srinivasan, P.R., Dulbecco, R. (1976). The Integrated State of Viral DNA in SV40-Transformed Cells. In: Schiminovich, S. (eds) The Biology of DNA Tumor Viruses. Milestones in Current Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6970-7_7
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DOI: https://doi.org/10.1007/978-1-4684-6970-7_7
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