Skip to main content
SpringerLink
Log in

The Role of CD8-Class I Interactions in CTL Function

  • Chapter
Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods

  • 131 Accesses

Abstract

With few exceptions, cytotoxic T lymphocytes (CTL) are activated to lyse cells that bear surface antigen displayed in the context of major histocompatibility complex (MHC) class I proteins. Although recognition of antigen per se is a function of the polymorphic T-cell receptor complex (TCR/CD3), expression of the CD8 glycoprotein correlates with restriction of the CTL to class I protein, and CD8 has been shown to interact with non-polymorphic residues in the class I α3 domain (Salter et al., 1990). Considerable evidence has now accumulated to demonstrate the critical role this interaction plays in adhesion and signal generation for CTL effector function (reviewed in Parnes, 1989).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Balk SP, Mescher MF (1981): Specific reversal of cytolytic T lymphocyte-target cell binding is induced by free target cells. J Immunol 127: 51–57

    Google Scholar 

  • Balk SP, Walker J, Mescher MF (1981): Kinetics of cytolytic T lymphocyte binding to target cells. J Immunol 126: 2177–2183

    Google Scholar 

  • Barber EK, Dev Dasgupta J, Schlossman SF, Trevllyan JM, Rudd CE (1989): The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56i°k) that phosphorylates the CD3 complex. Proc Natl Acad Sci USA 86: 3277–3281

    Article  Google Scholar 

  • Christinck ER, Luscher MA, Barber BH, Williams DB (1991): Peptide binding to class I MHC on living cells and quantitation of complexes required for CTL lysis. Nature 352: 67–70

    Article  Google Scholar 

  • Detmers PA, Wright SD, Olsen E, Kimball B, Cohn ZA (1987): Aggregation of complement receptors on human neutrophils in the absence of ligand. J Cell Biol 105: 1137–1145

    Article  Google Scholar 

  • Dustin ML, Springer TA (1989): T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature 341: 619–624

    Article  Google Scholar 

  • Garcia-Penarrubia P, Bankhurst AD (1989): Kinetic analysis of effector cell recylcing and effector-target binding capacity in a model of cell-mediated cytotoxicity. J Immunol 143: 2101–2111

    Google Scholar 

  • Goldstein SAN, Mescher MF (1987): Cytotoxic T cell activation by class I protein on cell-size artificial membranes: Antigen density and Lyt-2/3 function. J Immunol 138: 2034–2043

    Google Scholar 

  • Hahn WC, Rosenstein Y, Burakoff SJ, Bierer BE (1991): Interaction of CD2 with its ligand lymphocyte function-associated antigen-3 induces adenosine 3’,5’-cyclic monophosphate production in T lymphocytes. J Immunol 147: 14–21

    Google Scholar 

  • Irving BA, Weiss A (1991): The cytoplasmic domain of the T cell receptor chain is sufficient to couple to receptor-associated signal transduction pathways. Cell 64: 891–901

    Article  Google Scholar 

  • June CH, Fletcher MC, Ledbetter JA, Samelson LE (1990): Increases in tyrosine phosphorylation are detectable before phospholipase C activation after T cell receptor stimulation. J Immunol 144: 1591–1599

    Google Scholar 

  • Kane KP, Champoux P, Mescher MF (1989a): Solid-phase binding of class I and class II MHC proteins: Immunoassay and T cell recognition. Mol Immunol 26: 759–768

    Article  Google Scholar 

  • Kane KP, Goldstein SAN, Mescher MF (1988): Class I alloantigen is sufficient for cytolytic T lymphocyte binding and transmembrane signalling. Eur J Immunol 18: 1925–1929

    Article  Google Scholar 

  • Kane KP, Vitiello A, Sherman LA, Mescher MF (1989b): Cytolytic T lymphocyte response to isolated class I H-2 proteins and influenza peptides. Nature 340: 157–159

    Article  Google Scholar 

  • Larson RS, Hibbs ML, Springer TA (1990): The leukocyte integrin LFA-1 reconstituted by cDNA transfection in a nonhematopoietic cell line is functionally active and not transiently regulated. Cell Reg 1: 359–367

    Google Scholar 

  • Martz E (1976): Multiple target cell killing by cytolytic T lymphocytes and the mechanism of cytotoxicity. Transplantation 21: 5–11

    Article  Google Scholar 

  • Martz E (1977): Mechanism of specific tumor cell lysis by alloimmune T-lymphocytes: Resolution and characterization of discrete steps in the cellular interaction. Contemp Top Immunobiol 7: 301–361

    Google Scholar 

  • Martz E (1987): LFA-1 and other accessory molecules functioning in adhesions of T and B lymphocytes. Human Immunol 18: 3–37

    Article  Google Scholar 

  • Mescher MF, O’Rourke AM, Champoux P, Kane KP (1991): Equilibrium binding of cytotoxic T lymphocytes to class I antigen. J Immunol 147: 36–41

    Google Scholar 

  • Mustelin T, Coggeshall KM, Isakov N, Altman A (1990): T cell antigen receptor-mediated activation of phospholipase C requires tyrosine phosphorylation. Science 247: 1584–1587

    Article  Google Scholar 

  • Nakauchi H, Nolan GP, Hsu C, Huang HS, Kavathas P, Herzenberg LA (1985): Molecular cloning of Lyt2, a membrane glycoprotein marking a subset of mouse T lymphocytes: Molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions. Proc Natl Acad Sci USA 82: 5126–5130

    Article  Google Scholar 

  • O’Rourke AM, Mescher MF (1990): T-cell receptor-activated adhesion systems. Curr Opinion Cell Biol 2: 888–893

    Article  Google Scholar 

  • O’Rourke AM, Mescher MF (1992): Cytotoxic T lymphocyte activation involves a cascade of signalling and adhesion events. Nature 358: 253–255

    Article  Google Scholar 

  • O’Rourke AM, Apgar JR, Kane KP, Martz E, Mescher MF (1991): Cytoskeletal function in CD8- and T cell receptor-mediated interaction of cytotoxic T lymphocytes with class I protein. J Exp Med 173: 241–249

    Article  Google Scholar 

  • O’Rourke AM, Rogers J. Mescher MF (1990): Activated CD8 binding to class I protein mediated by the T-cell receptor results in signalling. Nature 346: 187–189

    Article  Google Scholar 

  • Panaccio M, Gillespie, MT, Walker ID, Kirszbaum L, Sharpe JA, Tobias GH, McKenzie IFC, Deacon NJ (1987): Molecular characterization of the murine cytotoxic T-cell membrane glycoprotein Ly-3 (CD8). Proc Natl Acad Sci USA 84: 6874–6878

    Article  Google Scholar 

  • Pardi R, Bender JR, Dettori C, Giannazza E, Engleman EG (1989): Heterogeneous distribution and transmembrane signalling properties of lymphocyte function-associated antigen (LFA-I) in human lymphocyte subsets. J Immunol 143: 3157–3166

    Google Scholar 

  • Parnes JR (1989): Molecular biology and function of CD4 and CD8. Adv Immunol 44: 265–311

    Article  Google Scholar 

  • Plaut M, Lichtenstein LM, Henney CS (1973): Studies on the mechanisms of lymphocyte mediated cytolysis III. The role of microfilaments and microtubules. J Immunol 110: 771–780

    Google Scholar 

  • Salter RD, Benjamin RJ, Wesley PK, Buxton SE, Garrett TP, Clayberger C, Krensky AM, Norment AM, Littmann DR, Parham P (1990): A binding site for the T-cell co-receptor CD8 on the x3 domain of HLA-A2. Nature 345: 41–46

    Article  Google Scholar 

  • Samelson LE, Phillips AF, Luong ET, Klausner RD (1990): Association of the fyn protein-tyrosine kinase with the T-cell antigen receptor. Proc Nat! Acad Sci USA 87: 4358–4362

    Article  Google Scholar 

  • Secrist JP, Karnitz L, Abraham RT (1991): T-cell antigen receptor ligation induced tyrosine phosphorylation of phospholipase C-7l. J Biol Chem 266: 12135–12139

    Google Scholar 

  • Shimizu Y, van Seventer GA, Horgan KJ, Shaw S (1990a): Regulated expression and binding of three VLA (ßl) integrin receptors on T cells. Nature 345: 250–253

    Article  Google Scholar 

  • Shimizu Y, van Seventer GA, Horgan KJ, Shaw S (1990b): Roles of adhesion molecules in T-cell recognition: Fundamental similarities between four integrins on resting human T cells (LFA-1, VLA-4, VLA-5, VLA-6) in expression, binding, and constimulation. Immunol Rev 114: 109–143

    Article  Google Scholar 

  • Shimizu Y, van Seventer GA, Horgan KJ, Shaw S (1990c): Costimulation of proliferative responses of resting CD4+ T cells by the interaction of VLA-4 and VLA-5 with fibronectin or VLA-6 with laminin. J Immunol 145: 59–67

    Google Scholar 

  • Sibley DR, Benovic JL, Caron MG, Lefkowitz RJ (1987): Regulation of transmembrane signalling by receptor phosphorylation. Cell 48: 913–922

    Article  Google Scholar 

  • Strom TB, Garovoy MR, Carpenter CB, Merrill JP (1973): Microtubule function in immune and non-immune lymphocyte mediated cytotoxicity. Science 181: 171–173

    Article  Google Scholar 

  • Ullrich A, Schlessinger J (1990): Signal transduction by receptors with tyrosine kinase activity. Cell 61: 203–212

    Article  Google Scholar 

  • van Kooyk Y, van de Wiel-van Kemenade P, Weder P, Kuijpers TW, Figdor CG (1989): Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes. Nature 342: 811–813

    Google Scholar 

  • van Kooyk Y, Weder P, Hogervorst F, Verhoeven AJ, van Seventer G, te Velde AA, Borst J, Keizer GD, Figdor CG (1991): Activation of LFA-I through a Ca’ + -dependent epitope stimulates lymphocyte adhesion. J Cell Biol. 112: 345–354

    Article  Google Scholar 

  • van Seventer GA, Shimizu Y, Horgan KJ, Shaw S (1990): The LFA-1 ligand ICAM-1 provides an important costimulatory signal for T cell receptor-mediated activation of resting T cells. J Immunol 144: 4579–4586

    Google Scholar 

  • Veillette A, Bookman MA, Horak EM, Bolen JB (1988): The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56. Cell 55: 301–308

    Article  Google Scholar 

  • Vitiello A, Potter TA, Sherman LA (1990): The role of ß2-microglobulin in peptide binding by class I molecules. Science 250: 1423–1426

    Article  Google Scholar 

  • Weiss A, Koretzky G, Schatzman RC, Kadlecek T (1991): Functional activation of the T-cell antigen receptor induces tyrosine phosphorylation of phospholipase Cil. Proc Natl Acad Sci USA 88: 5484–5488

    Article  Google Scholar 

  • Zagury D, Bernard J, Thierness N, Feldman M, Berke G (1975): Isolation and characterization of individual functionally reactive cytotoxic T lymphocytes: Conjugation, killing and recycling at the single cell level. Eur J Immunol 5: 818–822

    Article  Google Scholar 

Download references

Authors
  1. Anne M. O’Rourke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matthew F. Mescher
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of Immunology, Biochemistry and Immunopharmacology Unit, NIH-NIAID, 20892, Bethesda, Maryland, USA

    Michail V. Sitkovsky

  2. Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, 20892, Bethesda, Maryland, USA

    Pierre A. Henkart

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Birkhäuser Boston

About this chapter

Cite this chapter

O’Rourke, A.M., Mescher, M.F. (1993). The Role of CD8-Class I Interactions in CTL Function. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-6814-4_6

  • Publisher Name: Birkhäuser Boston

  • Print ISBN: 978-1-4684-6816-8

  • Online ISBN: 978-1-4684-6814-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation