Abstract
Determine the lytic functional activity of cytolysin (perform) in granule and cell extracts. Red cell lysis is measured by optical absorbance of supernatant hemoglobin.
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References
Henkart PA, Millard PJ, Reynolds CW, Henkart MP (1984): Cytolytic activity of purified cytoplasmic granules from cytotoxic rat LGL tumors. J Exp Med 160: 75
Millard PJ, Henkart MP, Reynolds CW, Henkart PA (1984): Purification and properties of cytoplasmic granules from cytotoxic rat LGL tumors. J Immunol 132: 3197
Yue CC, Reynolds CW, Henkart PA (1987): Inhibition of cytolysin activity in granules of large granular lymphocytes by lipids: Evidence for a membrane insertion mechanism of lysis. Mol Immunol 24: 647
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© 1993 Birkhäuser Boston
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Henkart, P. (1993). Measurement of Cytolysin Hemolytic Activity. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_49
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DOI: https://doi.org/10.1007/978-1-4684-6814-4_49
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4684-6816-8
Online ISBN: 978-1-4684-6814-4
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