Abstract
Tachyzoites of the ts-4 strain of T. gondii are maintained by in vitro passage in human foreskin fibroblasts at 33°C. T. gondii soluble antigens (STAg) are prepared from parasites obtained by peritoneal lavage, 3 days after infection of Swiss Webster mice with 5–10 × 106 RH strain tachyzoites. Tachyzoites are concentrated by centrifugation (1500 × g, 10 min) and repeatedly sonicated until no intact parasites remain. After centrifugation at 10,000 × g for 30 min, the supernatant is collected, dialyzed against PBS, and stored at −80°C.
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© 1993 Birkhäuser Boston
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Gazzinelli, R.T., Denkers, E., Hakim, F., Sher, A. (1993). Protocol for Assaying CTL Activity Against Toxoplasma gondii. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_47
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DOI: https://doi.org/10.1007/978-1-4684-6814-4_47
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4684-6816-8
Online ISBN: 978-1-4684-6814-4
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