Abstract
Conventional assays used for the measurement of cell-mediated cytotoxicity involve interactions between effector lymphocytes and antigen-bearing targets maintained and/or manipulated into a suspension culture. However, in vivo,immunologic reactions also involve T lymphocytes responding to targets that are bound to each other or to an extracellular matrix. To that end, we have developed an in vitro murine model, which more closely mimics such an in vivo situation, to explore the functional consequences that result from T cell-tissue or tumor interaction (Russell et al., 1988).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abrams SI, McCulley DE, Meleedy-Rey P, Russell JH (1989): Cytotoxic T lymphocyte (CTL)-induced loss of target cell adhesion and lysis involve common and separate CTL signaling pathways. J Immunol 142: 1789–1796
Abrams SI, Russell JH (1991): CD4+ T lymphocyte-induced target cell detachment. A model for T cell-mediated lytic and nonlytic inflammatory processes. J Immunol 146: 405–413
Abrams SI, Russell JH (1991): CD4+ T lymphocyte-induced target cell detachment. A model for T cell-mediated lytic and nonlytic inflammatory processes. J Immunol 146: 405–413
Russell JH, Musil L, McCulley DE (1988): Loss of adhesion. A novel and distinct effect of the cytotoxic T lymphocyte-target interaction. J Immunol 140: 427–432
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Birkhäuser Boston
About this chapter
Cite this chapter
Abrams, S.I., Russell, J.H. (1993). Target Cell Detachment Assay. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_46
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6814-4_46
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4684-6816-8
Online ISBN: 978-1-4684-6814-4
eBook Packages: Springer Book Archive