Abstract
The nodulation of peas and vetches by R. leguminosarum biovar viciae requires the expression of several bacterial nodulation (nod) genes (1). One of these (nodD) is expressed constitutively and is involved in the positive regulation of the other nod genes (2). Flavonoids such as naringenin and eriodictyol are secreted from pea roots (3,4) and appear to be recognized by the NodD protein (5,6) which is then involved in the activation of transcription of the other nod genes. This regulation is mediated by the NodD protein binding to the promoters of the other nod genes (7) at a highly conserved DNA sequence (nod-box). In R. meliloti there also appears to be a repressor of nod gene expression (8) but we have not found such a repressor in b.v. viciae. However, we have identified mutations that lower the level of induction of nod gene expression. Recombinant plasmids which suppress these mutations have been identified and DNA hybridization using these clones has shown that they are from outside the nod gene region.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Downie, J.A. & Johnston, A.W.B. (1988) Plant Cell Environ. 11, 403–412.
Rossen, L., Shearman, C.A., Johnston, A.W.B. & Downie, J.A. (1985) EMBO 4, 3369–3373.
Zaat, S.A.J., Wijffelman, C.A., Mulders, I.H.M., van Brussel, A.A.N. & Lugtenberg, B.JJ. (1988) Plant Physiol 86, 1298–1303.
Firmin, J.L., Wilson, K.E., Rossen, L. & Johnston, A. (1986) Nature 324, 90–92.
Burn, J.E., Hamilton, W.D., Wootton, J.C. & Johnston, A.W.B. (1989) Mol. Microbiol. 3, 1567–1577.
Spaink, H.P., Wijffelman, C.A., Pees, E., Okker, R.J.H. & Lugtenberg, B.J.J. (1987) Nautre 328, 337–339.
Hong, G-F., Burn, J. & Johnston, A.. (1987) Nucl. Acids. Res. 15, 9677–9690.
Kondorosi, E., Gyuris, J., Schmidt, J., John, M., Duda, E., Hoffmann, B., Schell, J. & Kondorosi, A. (1989) EMBO J. 8, 1331–1340.
Downie, J.A., Knight, C.D., Johnston, A.W.B. & Rossen, L. (1985) Mol. Gen. Genet. 198, 255–262.
Wijffelman, C.A., Pees, E., can Brussel, A.A.N., Okker, R.J.H. & Lugtenberg, B.J.J. (1985) Arch. Microbiol. 143, 225–232.
Shearman, C.A., Rossen, L., Johnston, A.W.B. & Downie, J.A. (1986) EMBO 5, 647–652.
Surin, B.P. & Downie, J.A. (1988) Mol. Microbiol. 2, 173–183.
Surin, B.P., Watson, J.M., Hamilton, W.D.O., Economou, A., Downie, J.A. (1990) Mol. Microbiol. 4, 245–252.
Economou, A., Hawkins, F.K.L., Downie, A. J. & Johnston, A.W.B. (1989) Mol. Microbiol. 3 (1), 87–93.
De Maagd, R.A., Spaink, H.P., Pees, E., Mulders, I. H. M., Wijfjes, A., Wijffelman, C.A., Okker, R.J.H. & Lugtenberg, B.J.J. (1989a) J. Bacteriol. 171, 1151–1157.
Canter-Cremers, H.C.J., Wijffelman, C.A., Pees, E., Rolfe, B.G., Djordjevic, M.A. & Lugtenberg, B.J.J. (1988) J. Plant Physiol. 132, 398–404.
Borthakur, D., Barber, C.E., Lamb, J.W., Daniels, M.J., Downie, J.A. & Johnston, A.W.B. (1986) Mol. Gen. Genet. 200, 278–282.
Phillip-Hollingsworth, S., Hollingsworth, R.I. & Dazzo, F. (1989) J. Biol. Chem. 264, 1461–1466.
Surin, B.P. & Downie, A.J. (1989) Plant Mol. Biol. 12, 19–29.
Spaink, H.P., Wiffelman, C.A., Pees, E., Okker, R.J.H. & Lugtenberg, B.J.J. (1987) Nature 328, 337–339.
Bibb, M.J., Biro, S., Motamedi, H., Collins, J.F. & Hutchinson, CR. (1989) EMBO J. 8, 2727–2736.
Downie, J.A. (1989) Mol. Microbiol. 3, 1649–1651.
Zaat, S.A.J., Van Brussel, A.A.N., Tak, T., Pees, E. & Lugtenberg, B. J. (1987) J. Bacteriol. 169, 3388–3391.
Lerouge, P., Roche, P., Faucher, C., Maillet, F., Truchet, G., Promé J.E. & Dénarié, J. (1990) Nature 344, 781–784.
Raetz, C.R.H. (1986) Ann. Rev. Genet. 20, 253–295.
Economou, A., Hamilton, W.D.O., Johnston, A.W.B. & Downie, J.A. (1990) EMBO J. 9, 349–354.
De Maagd, R.A., Wijfjes, A.H.M., Spaink, H.P., Ruiz-Sainz, J.E., Wijffelman, C.A., Okker, R.J.H. & Lugtenberg, B.J.J. (1986b) J. Bacteriol. 171, 6746–6770.
Holland, I.B., Wang, R., Seror, S.J. & Blight, M. (1989) Symp. Soc. Gen. Micro. 44, 219–254.
Létoffé, S., Delepelaire, P. & Wandersman, C. (1990) EMBO J. 9, 1375–1382.
Rossen, L., Johnston, A. & Downie, J.A. (1984) Nucl. Acids Res. 12, 9497–9508.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1990 Routledge, Chapman & Hall, Inc.
About this chapter
Cite this chapter
Downie, J.A. et al. (1990). The Rhizobium leguminosarum bv. viciae NodO protein compensates for the exported signal made by the host-specific nodulation genes. In: Gresshoff, P.M., Roth, L.E., Stacey, G., Newton, W.E. (eds) Nitrogen Fixation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6432-0_21
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6432-0_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6434-4
Online ISBN: 978-1-4684-6432-0
eBook Packages: Springer Book Archive