Abstract
To address controversies concerning the effects of β;-adrenergic stimulation on the rate of myocardial cross-bridge cycling, we measured three mechanical variables, isometric tension development, transient tension response to a step stretch in length (<1% of muscle length), maximum velocity of shortening, and a chemical variable, ATPase activity before and after treatment with the catalytic subunit of protein kinase A (PKA) in demem-branated rat right ventricular trabeculae, and also measured three mechanical variables before and after treatment with D-cAMP in intact ryanodine-induced tetanized preparations. PKA treatment (1 U/μl, 40 min) shifted the pCa-tension relation to the right from 5.41 to 5.26 at pCa50 (the [Ca2+] required for half maximal steady tension) without changing the steepness of the pCa-tension relation and the maximum tension. The rate of the transient tension changes was significantly increased after either PKA or D-cAMP treatment (5 mM, 15 min), regardless of the level of isometric tension. Vmax was increased for a given Ca2+ concentration after either the PKA or D-cAMP treatment, despite the reduced level of isometric tension. The PKA treatment also shifted the pCa-ATPase activity to the right slightly from 5.47 to 5.40 at pCa50, but increased the ATPase activity during a given level of steady isometric tension generation, resulting in an increased tension cost (ATPase activity/tension). These results suggest that, in rat right ventricular trabeculae, β-adrenergic stimulation may increase the rate of cross-bridge cycling by increasing the rate of cross-bridge detachment from actin through a PKA-mediated mechanism, although PKA reduces the Ca2+-sensitivity of the contractile system.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Katz, A.M. Adv. Cyc. Nucl. Res. 11, 303–343 (1979).
Winegrad, S. Circ. Res. 55, 565–574 (1984).
Berman, M.R., Peterson, J.N., Yue, D.T. & Hunter, W.C. J. Mol. Cell. Cardiol. 20, 415–426 (1988).
Hoh, J.F.Y., Rossmanith G.H., Kwan, L.J. & Hamilton A. M. Circ. Res. 62, 452–461 (1988).
Huxley, A.F. Prog. Biophys. Chem. 7, 255–318 (1957).
Siemankowski, R.F., Wiseman, M.O. & White, H.D. Proc. Natl. Acad. Sci. USA. 82, 658–662 (1985).
Saeki, Y., Shiozawa, K., Yanagisawa, K. & Shibata T. J. Mol. Cell. Cardiol. 22, 453–460 (1990).
Saeki, Y., Shiozawa, K., Paik, C-H. & Yanagisawa, K. J. Muscle Res. Cell Motil. 12, 152–160 (1991).
Chiu, Y.C., Walley, K.R. & Ford, L.E. Circ. Res. 65, 1161–1171 (1989).
de Tombe, P.P. & ter Keurs, H.E.D.J. Circ. Res. 68, 382–391 (1991).
Strang, K.T., Sweitzer, N.K., Greaser M.L. & Moss, R.L. Circ. Res., 74, 542–549 (1994).
Edman, K.A.P. J. Physiol. 291, 143–150 (1979).
Garvey, J.L., Kranias, E.G. & Solaro, R.J. Biochem. J. 249, 709–714 (1988).
Hofmann, P.A. & Lange J.H., Circ. Res. 74, 718–726 (1994).
de Tombe, P.P. & Stienen, G.J.M. Circ. Res. 76, 734–741 (1995).
Barsotti, R.J. & Ferenczi, M.A. J. Biol. Chem. 365, 16750–16756 (1988).
Yue, D.T., Marban, E. & Wier, W.G. J.Gen.Physiol. 87, 223–242 (1986).
Winegrad, S., Weisberg, A., Lin L.E. & McClellan, G. Circ. Res. 58, 83–95 (1986).
Alpert, N.R., Mulieri, L.A. & Hasenfuss, G. in The Heart and Cardiovascular System: Scientific Foundations, 2nd ed. (eds. Fozzard H.A., Jennings, R.B., Haber, E. & Kazs A.M.) 111–128 (Raven Press Publishers, New York, 1991)
Holubarsch, C.H., Hasenfuss, G. Just, H., Blanchard, E.M., Mulieri, L.A. & Alpert, N.R. Cardioscience 1, 33–41 (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Saeki, Y., Kobayashi, T., Takigiku, K., Sugi, H. (1998). Influences of Protein Kinase A and D-Camp on Actin-Myosin Interaction and Energy Consumption of Cardiac Muscles. In: Sugi, H., Pollack, G.H. (eds) Mechanisms of Work Production and Work Absorption in Muscle. Advances in Experimental Medicine and Biology, vol 453. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6039-1_51
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6039-1_51
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6041-4
Online ISBN: 978-1-4684-6039-1
eBook Packages: Springer Book Archive