Calcium Dependence of the Apparent Rate of Force Generation in Single Striated Muscle Myofibrils Activated by Rapid Solution Changes

Abstract

Single myofibrils or small groups of myofibrils were isolated from different types of striated muscle: rabbit psoas, frog tibialis anterior, frog atrial and ventricular muscle. The Ca²⁺ concentration of the solution perfusing the myofibrils was changed within few milliseconds by translating the interface between two flowing streams of solution across the preparations. In all types of myofibrils tested, the time course of force rise in response to maximal activation (pCa 4.75) was approximately monoexponential and nearly superimposable on that observed after a release-restretch protocol applied to the myofibril at the plateau of maximal contractions. This suggests that the kinetics of force development following rapid myofibril activation essentially reflects the kinetics of interaction between contractile proteins. The half time of force rise in response to maximal activation varied among different myofibril types; it was shortest in frog tibialis anterior myofibrils and longest in frog ventricular myofibrils. In all types of myofibril preparations tested the half time of force rise increased with decreasing Ca²⁺ levels in the activating solution. The finding provides support for a kinetic mechanism of force regulation by Ca²⁺ in all types of striated muscle. The extent of this Ca²⁺ effect, however, varied among the different myofibril preparations tested; at 15°C for instance, it was smaller in frog tibialis anterior myofibrils than in the other preparations.