Studies on the 14.5 nm Meridional X-Ray Diffraction Reflection During Length Changes of Intact Frog Muscle Fibres

Abstract

The intensity of the 14.5 nm meridional reflection (M3) from activated skeletal muscle fibres was studied in both single fibres and fibre bundles during the imposition of length changes. During shortening at small load, the intensity of the reflection decreased within 2 ms to less than 20% of isometric intensity, then recovered partially during the remainder of the shortening. When shortening was terminated, recovery of intensity was delayed. Small shortening steps (0.5% fibre length) produced a fall in M3 intensity (I_M3) delayed by ca. 250 us compared to the fall in tension. For larger step releases (1% fibre length), the fall in I_M3 was not delayed. The fall in I_M3 could be almost completely reversed by a subsequent restretch applied within 1.5 ms. Beyond 10 ms after the initial release, the restretch caused a further fall in intensity. A rapid step stretch (0.5% fibre length) also caused a fall in I_M3 without delay, which was partially reversed by a release applied within 10 ms. A second small release applied 3 ms (or less) after the first caused a second fall in M3 intensity, but without delay and with faster time course. Small amplitude sinusoidal length oscillations (0.15−0.2% sarcomere; 1 kHz) caused a sinusoidal change in M3 intensity, which was 180° out of phase with the force oscillations, and lacked distortion during its release phase.