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Products of Inflammatory Cells Synergistically Enhance Superoxide Production by Phagocytic Leukocytes

  • Chapter
Cell-Cell Interactions in the Release of Inflammatory Mediators

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 314))

Abstract

Superoxide (O −2 ) is a major component of the oxygen-dependent antimicrobial and cytocidal arsenal of neutrophils 1, 2. The oxidase system that generates this substance is dormant and disassembled in unstimulated cells and consists of both membrane-bound and soluble (“cytosolic factors”) components3, 4. The known membrane-components are a low-potential, heterodimeric b-cytochrome5, 6 and a ras-related GTP-binding protein7. The most thoroughly characterized cytosolic factors are proteins with molecular masses of 47 (p47) and 67kDa8–11. Upon stimulation of neutrophils, there is a translocation of the soluble components to the plasmalemma where the oxidase is assembled12, 13 (Figure 1). This assembly requires the presence of the b-cytochrome12, 14 and is associated with and/or organized by cytoskeletal proteins15. The intact system produces O −2 according to the following stoichiometry:

$${\rm{NAPDH + 2}}{{\rm{0}}_{\rm{2}}} \to {\rm{2}}{{\rm{0}}_{\rm{2}}}^{\rm{ - }}{\rm{ + NAD}}{{\rm{P}}^{\rm{ + }}}{\rm{ + 2}}{{\rm{H}}^{\rm{ + }}}{\rm{.}}$$

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Authors and Affiliations

  1. Department of Cell Physiology, Boston Biomedical Research Institute, USA

    John A. Badwey & Jiabing Ding

  2. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA

    John A. Badwey

  3. Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA, USA

    Paul G. Heyworth

  4. Department of Cell Biology, Neurobiology and Anatomy, Program in Molecular, Cellular and Developmental Biology and the Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA

    John M. Robinson

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  1. New York Medical College, Valhalla, New York, USA

    Patrick Y.-K. Wong

  2. Harvard Medical School, Boston, Massachusetts, USA

    Charles N. Serhan

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Badwey, J.A., Ding, J., Heyworth, P.G., Robinson, J.M. (1991). Products of Inflammatory Cells Synergistically Enhance Superoxide Production by Phagocytic Leukocytes. In: Wong, P.YK., Serhan, C.N. (eds) Cell-Cell Interactions in the Release of Inflammatory Mediators. Advances in Experimental Medicine and Biology, vol 314. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6024-7_2

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