A Yeast Expression System and Site-Directed Mutagenesis of a Fungal Aspartic Proteinase, Mucor Rennin
The cheese industry has long used a characteristic aspartic proteinase, chymosin (calf rennin) obtained from the calf stomach, as a milk coagulant for cheese making. A severe shortage of chymosin in 1950 stimulated efforts to find substituting microbial enzymes and Arima et al. succeeded in discovering such an enzyme from a fungal strain, Mucor pusillus.1 A similar enzyme was subsequently found from a closely related species, Mucor miehei,2 and these fungal aspartic proteinases are called Mucor rennin. Currently more than a half of cheese in the world is produced with Mucor rennin.
KeywordsAspartic Proteinase Recombinant Yeast High Proteolytic Activity Milk Clotting Activity Yeast Expression System
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- 2.M. Ottensen, and W. Richert, The isolation and partial purification of an acid protease produced by Mucor miehei, C. R. Trav. Lab. Carlsberg 37: 301 (1970).Google Scholar
- 20.A. Sali, B. Veerapandian, J. B. Cooper, S. I. Foundling, D. J. Hoover, and T. L. Blundell, High-resolution X-ray diffraction study of the complex between endothiapepsin and an oligopeptide inhibitor: the analysis of the inhibitor binding and description of the rigid body shift in the enzyme, EMBO J. 8: 2179 (1989).PubMedGoogle Scholar