Production of Prochymosin in Lactococci

  • Guus Simons
  • Ger Rutten
  • Miranda Hornes
  • Monique Nijhuis
  • Martien van Asseldonk
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)


Lactococci (Lactococcus lactis ssp. lactis and L. lactis ssp. cremoris) are used on a large scale as starter cultures for the production of fermented milk products such as cheese, butter and buttermilk. Lactoccocci are cultured in milk based media and, in addition to the milk clotting agent chymosin, are added directly to the cheese vat during cheese manufacturing. It is interesting to use lactococci as a production organism for chymosin since no foreign components or proteins originating from media or the host are added to the cheese vat when chymosin isolated from lactococci is used for cheese manufacturing.


Leader Sequence Strain MG13639 Fermented Milk Product Cheese Manufacturing Foreign Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    T. D. Thomas and G. G. Pritchard, FEMS Microbiol. Rev. 46: 245–268 (1987).CrossRefGoogle Scholar
  2. 2.
    J. Kok, K. Leenhouts, A. J. Haandrikman, A. M. Ledeboer and G. Venema, Appl. Environ. Microbiol. 54: 231–238 (1985).Google Scholar
  3. 3.
    P. Vos, G. Simons, R. J. Siezen and W. M. de Vos, J. Biol. Chem. 264: 13579–13585 (1989).PubMedGoogle Scholar
  4. 4.
    M. Kiwaki, H. Ikemura, M. Shimizu-Kadota and A. Hiroshima, Mol. Microbiol. 3: 359–369 (1989).PubMedCrossRefGoogle Scholar
  5. 5.
    A. J. Haandrikman, J. Kok, H. Laan, A. Soemitro, A. M. Ledeboer, W. Konings and G. Venema, J. Bacteriol. 171: 2789–2794 (1989).PubMedGoogle Scholar
  6. 6.
    P. Vos, M. van Asseldonk, F. van Jeveren, R. J. Siezen, G. Simons and W. M. de Vos, J. Bacteriol. 171: 2795–2802 (1989).PubMedGoogle Scholar
  7. 7.
    G. Simons, M. van Asseldonk, G. Rutten, M. Nijhuis, M. Hornes and W. M. de Vos, 5th European Congress on Biotechnology, Copenhagen, Denmark. Munksgaard International Publisher, Copenhagen, in press (1990).Google Scholar
  8. 8.
    G. von Heijne and L. Abrahamsen, FEBS Lett. 244: 439–446 (1989).CrossRefGoogle Scholar
  9. 9.
    M. J. Gasson, J. Bacteriol 154: 1–9 (1983).PubMedGoogle Scholar
  10. 10.
    G. Simons, M. Nijhuis, A. Braks, G. Rutten and M. van Asseldonk, in preparation (1990).Google Scholar
  11. 11.
    W. M. De Vos, FEMS Microbiol. Rev. 46: 281–295 (1987).Google Scholar
  12. 12.
    A. E. Franke, F. S. Kaczmarek, M. E. Eisenhard, K. F. Geoghegan, D. E. Danley, J. R. De Zeeuw, M. M. O’Donnell, M. G. Gollaher, Jr. and L. S. Davidow, Dev. in Industrial Microbiology, 29: 43–57 (1988).Google Scholar
  13. 13.
    M. Ward, L. J. Wilson, K. H. Kodama, M. W. Rey and R. M. Berka, Bio/Technology 8: 435–440 (1990).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Guus Simons
    • 1
  • Ger Rutten
    • 1
  • Miranda Hornes
    • 1
  • Monique Nijhuis
    • 1
  • Martien van Asseldonk
    • 1
  1. 1.Molecular Genetics Group, Department of Biophysical ChemistryNetherlands Institute for Dairy Research (NIZO)EdeThe Netherlands

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