Abstract
When the unicellular photosynthetic alga Chlamydomonas is faced with changes in direction, wavelength or intensity of the ambient light, it exhibits distinct behavioral responses, which are direction changes if the changes are small or stop responses upon larger changes. At continuous irradiation Chlamydomonas orients to or away from the light source (positive or negative phototaxis). These movement responses have been under investigation for more than 100 years (Pfeffer, 1904; Nultsch and Häder, 1988) but neither the photoreceptor nor the signal transduction process have been characterized in detail.
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References
Beckmann, M., and Hegemann, P., 1991, In vitro identification of rhodopsin in the green alga Chlamydomonas, Biochemistry, submitted.
Dolle, R., Pfau, J., and Nultsch, W., 1986, Role of calcium ions on motility and phototaxis on Chlamydomonas reinhardtii, J. Plant Physiol., 126:467.
Foster, K. W., Saranak, J., Derguini, F., Rao, J., Zarrilli, G. R., Okabe, M., Fang, J.-M., Shimizu, N., and Nakanishi, K., 1988b, Rhodopsin activation: A novel view suggested by in vivo Chlamydomonas experiments, J. Am. Soc., 110:6588.
Foster, K. W., Saranak, J., Derguini, F., Zarrilli, G., Johnson, R., Okabe, M., and Nakanishi, K., 1989, Activation of Chlamydomonas rhodopsin in vivo does not require isomerization of retinal., Biochemistry, 28:819.
Foster, K. W., Saranak, J., Patel, N., Zarrilli, G., Okabe, M., Kline, T., and Nakanishi, K., 1984, A rhodopsin is the functional chromophore for phototaxis in the unicellular alga Chlamydomonas, Nature, 311:756.
Foster, K. W., Saranak, J., and Zarrilli, G. R., 1988a, Autoregulation of rhodopsin synthesis in Chlamydomonas reinhardtii, Proc. Natl. Acad. Sci. USA, 85:6379.
Foster, K. W., and Smyth, R. D., 1980, Light antennas in phototactic algae, Microbiol. Rev., 44:572.
Harz, H., and Hegemann, P., 1991, Rhodopsin-regulated ion channel activation in Chlamydomonas, Biophys. J., submitted.
Hegemann, P., and Bruck B., 1989, The light-induced stop response in Chlamydomonas. Occurence and adaptation phenomena, Cell Motil., 14:501.
Hegemann, P., Hegemann, U., Foster, K. W., 1988, Reversible bleaching of Chlamydomonas reinhardtii rhodopsin in vivo, Photochem. Photobiol., 48:123.
Hegemann, P., Neumeier, K., Hegemann, U., and Kuehnle, E., 1990, The role of calcium in Chlamydomonas movement responses as analysed by calcium channel inhibitors, Photochem. Photobiol., 52:575.
Kamyia. R., and Witman, G., 1984, Submicromolar levels of calcium control the balance of beating between the two flagella in demembrenated models of Chlamydomonas., J. Cell Biol., 98:97.
Korolkov, S. N., Garnovskaya, M. N., Basov, A. S., Chunaev, A. S., and Dumler, I. L., 1990, The detection and characterisation of G-proteins in the eyespot of Chlamydomonas reinhardtii, FEBS Lett., 270:132.
Krinski, N. I., and Litvin, R. P., 1964, Carotenoids of wild type and mutant strains of the green alga Chlamydomonas reinhardl, Plant Physiol,. 48:680.
Litvin, F. F., Seneshchekov, O. A., and Seneshchekov, V. A., 1978, Photoreceptor electric potential in the phototaxis in the alga Haematococcus pluvialis, Nature, 271:476.
Nakanishi, K., Derguini, F., Rao, J., Zarrilli, G., Okabe, M., Lien, T., Johnson, R., Foster, K. W., and Saranak, J., 1989, Theory of rhodopsin activation: probable charge redistribution of excited state chromophore, Pure Appl. Chem. 61:361.
Nultsch, W., and Häder, D.-P., 1988, Photomovement in motile microorganisms n, Photochem. Photobiol., 47:837.
Nultsch, W., Pfau, J., and Dolle, R., 1986, Effect of calcium channel blockers on phototaxis and motility of Chlamydomonas reinhartii, Arch. Microbiol., 144:393.
Nultsch, W., Throm, G., v. Rimscha, I., 1971, Phototaktische Untersuchungen an Chlamydomonas reinhardtii Dangeard in homo-kontinuierlicher Kvltur, Arch. Microbiol. 80:351.
Oesterhelt, D., Hegemann, P., Tavan, P., and Schulten, K., 1986, Trans-cis isomerization of retinal and a mechanism for ion translocation in halorhodopsin, Eur. J. Biophys., 14:123.
Pfeffer, W., 1904, “Pflanzenphysiologie,” Leipzig.
Schmidt, J. A., and Eckert, R., 1976, Calcium couples flagellar reversal to photostimulation in Chlamydomonas reinhardtii., Nature, 262:113.
Smith, S. O., Paling, I., Copie, V., Raleigh, D. P., Courtin, J., Pardoen, J. A., Lugtenburg, J., Mathies, R. A., and Griffin, R. G., 1987, Low temperature solid state NMR studies of retinal chromophore in rhodopsin, Biochemistry, 26:1606.
Stavis, R. L., and Hirschberg, R., 1973, Phototaxis in Chlamydomonas reinhardtii, J. Cell Biol., 59:367.
Takahashi, T., Yan, B., Mazur, P., Derguini, F., Nakanishi, K., and Spudich, J., 1990, Color regulation in the archibacterial phototaxis receptor phoborhodopsin (sensory rhodopsin II), Biochemistry in press.
Uhl, R., and Hegemann, P., 1990a, Adaptation of Chlamydomonas phototaxis: I. A light scattering apparatus for measuring the phototactic rate of microorganisms with high time resolution, Cell Motil., 15:230.
Uhl, R., and Hegemann, P., 1990b, Probing visual transduction in a plant cell. Optical recording of rhodopsin-induced structural changes from Chlamydomonas, Biophys. J., in press.
van der Steen, R., Biesheuvel, P. L., Mathies, R. A., and Lugtenburg, J., 1986, Retinal analogs with locked 6–7 conformation show that bacteriorhodopsin requires the 6-s-trans conformation, J. Am. Chem. Soc., 108:6410.
Warshel, A., and Karplus, M., 1974, Calculation of ππ* excited state conformations and vibronic structure of retinal and related molecules, J. Am. Chem. Soc., 96:5677.
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© 1991 Plenum Press, New York
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Hegemann, P. (1991). Photoreception in Chlamydomonas. In: Lenci, F., Ghetti, F., Colombetti, G., Häder, DP., Song, PS. (eds) Biophysics of Photoreceptors and Photomovements in Microorganisms. NATO ASI Series, vol 211. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5988-3_17
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DOI: https://doi.org/10.1007/978-1-4684-5988-3_17
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