Summary
The appearance of band 3 structural modifications related to aging could be evidenced by means of monoclonal antibodies against senescence antigen. Hence in the attempt to provide an immunological marker of erythrocyte aging, we raised a monoclonal antibody against native band 3 (B6 MoAb), which seems to detect differences in the band 3 molecule from erythrocytes of different ages separated by density gradient.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
D. M. Lieberman, and R. A. F. Reithmeier, Localization of Carboxyl terminus of band 3 to cytoplasmic side of the erythrocyte membrane using antibodies raised against a synthetic peptide, J. Biol. Chem. 263:10022 (1988).
P. K. K. Tai, and Carter-Su, Monoclonal antibody to the human glucose transports that differentiates between the glucose and nucleoside transports, Biochemistry 27:6062 (1988).
S. D. Wainwright, M. J. A. Tanner, G. E. M. Martin, J. E. Yandle, and C. Holmes, Monoclonal antibodies to the membrane domain of the human transport protein, Biochem. J. 258:211 (1989).
M. Czerwinski, K. Wasniowska, I. Steuden, M. Duk, A. Wiedlocha, and E. Lisowska, Degradation of the human erythrocyte membrane band 3 studied with monoclonal antibody directed againist an epitope on the cytoplasmic fragment of band 3, Eur. J. Biochem. 174:647 (1988).
M. M. B. Kay, K. Sorenson, P. Wong, and P. Bolton, Antigenicity, storage and aging: physiologic antibodies to cell membrane and serum proteins, Mol. Cell. Biochem. 49:65 (1982).
M. M. B. Kay, Band 3, the predominant transmembrane polypeptide undergoes proteolytic degradation as cells age, Monog. Dev. Biol. 17:245 (1984).
A. Giuliani, L. Ferroni, P. Caprari and A. M. Salvati, Band 3 purification: comparative evaluation of different extraction systems, Clin. Chem. Enzym. Comms. 3:299 (1990).
E. Beutler, K. G. Blume, J. C. Kaplan G. W. Lohr, B. Ramot and W. N. Valentine, International Committee for Standardization in Haematology: recommended methods for red cell enzyme analysis, Br. J. Haematol. 35:331 (1977).
G. Salvo, P. Caprari, P. Samoggia, G. Mariani and A. M. Salvati, Human erythrocyte separation according to age on a discontinuous “Percoll” density gradient, Clin. Chim. Acta 122:293 (1982).
S. Marini, G. Citro, S. di Cesare, R. Zito and B. Giardina, Production and characterization of monoclonal antibodies against DNA single ring diamine adducts, Hybridoma 7:193 (1988).
U. K. Laemmli, Cleavage of structural proteins by bacteriofage T4, Nature 227:680 (1979).
W. N. Burnette, “Western Blotting”: electrophoretic transfer of proteins from sodium-dodecyl sulphate Polyacrylamide gel electrophoresis to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein A, Anal. Biochem. 112:195 (1981).
M. M. B. Kay, Localization of senescent antigen on band 3, Proc. Natl. Acad. Sci. N. Y. 81:5753 (1984).
M. L. Jennings, M. P. Anderson and R. Monaghan, Monoclonal antibodies against human erythrocyte band 3 protein J. Biol. Chem. 261:9002 (1986).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Ferroni, L. et al. (1991). A New Monoclonal Antibody to an Age Sensitive Band 3 Transmembrane Segment. In: Magnani, M., De Flora, A. (eds) Red Blood Cell Aging. Advances in Experimental Medicine and Biology, vol 307. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5985-2_31
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5985-2_31
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5987-6
Online ISBN: 978-1-4684-5985-2
eBook Packages: Springer Book Archive